Effect of smear layer pretreatment with chemical methods on bond strength of universal adhesives

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Thanawat Ruaydee
Vanthana Sattabanasuk
Pipop Saikaew


Objective: The purpose of this study was to evaluate effect of smear layer pretreatment with 2.5% sodium hypochlorite solution followed by p-toluenesulfinic acid sodium salt (Accel) and 17% EDTA solution on microtensile bond strength (µTBS) of two universal adhesives

Materials and Methods: Forty-eight extracted third molars were used in this study. The crown of tooth was sectioned to exposed dentin. Smear layer was created by medium grit diamond bur with high speed aerotor under coolant for 5 strokes. Smear layer modifications were performed in different experimental groups: No treatment (G1), etching phosphoric acid for 15 s (G2), agitation with 17%EDTA solution using microbrush for 30 s (G3) and agitation with 2.5% Sodium hypochlorite solution using microbrush for 60 s followed by Accel application 5 s (G4). All bond Universal (ABU) and Clearfil Universal Bond Quick (CUQ) were used in this study. The microtensile bond strength test was performed on different smear layer pretreatment and universal adhesives (n=6). Data were analyzed using two-way ANOVA and Duncan multiple comparison test at the 95% significant level with SPSS software.

Results: Both adhesive systems and surface treatments had influence on microtensile bond strength. For ABU, the µTBS values were as followed G2>G3,G4>G1, respectively. Whereas the data for CUQ were G3,G4>G1,G2, respectively.  

Conclusion: Smear layer pretreatment with 17%EDTA agitation for 30 s or 2.5% NaOCl agitation for 60 s followed by Accel application improve immediate µTBS for both universal adhesives.


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Ruaydee T, Sattabanasuk V, Saikaew P. Effect of smear layer pretreatment with chemical methods on bond strength of universal adhesives. M Dent J [Internet]. 2021 Jun. 25 [cited 2023 Dec. 11];41(Suppl):S1-S5. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/250599
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