Effect of 30-day toothbrushing on permeability of desensitizer occluded root dentin: In vitro study

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Yossaya Laemlak
Pisol Senawongse
Pipop Saikaew


Objective: This study aims to evaluate dentin permeability after treating with desensitizers under toothbrushing for 30 days and to analyze which method would give the most sustainable plugged dentinal tubules.

Materials and Methods: Root dentin disc with approximate thickness of 0.4-0.6 mm prepared from upper premolars were immersed with 17% EDTA solution to gain maximum dentin permeability. The specimens were randomly divided into 4 groups; positive control (Er,Cr:YSGG laser), negative control (no treatment), brushing with Colgate® Sensitive ProRelief™ toothpaste and dab-on with Colgate® Sensitive ProRelief™ toothpaste. The second dentin permeability was assessed and then all the specimens were subjected for toothbrushing with total 1200 strokes to simulate daily toothbrushing for 30 days with brushing force 200 g and frequency 120 strokes/min. Then, dentin permeability was re-evaluated after brushing procedure. The specimens were randomly selected from each group for dentinal surface observation under SEM. Data were analyzed with 2-way ANOVA and Dunnett T3 multiple comparison.

Results: Significant reduction of dentin permeability was found after dentin were treated with Er,Cr:YSGG laser, brushing with Colgate® Sensitive ProRelief™ toothpaste or dab-on with Colgate® Sensitive ProRelief™ toothpaste (p<0.05). No significant difference of dentin permeability among three treated groups was found (p≥0.05). The brushing after treatment had no effect to the permeability of treated specimens.

Conclusion: All three desensitizers were able to reduce dentin permeability and had ability to maintain the effectiveness up to 30 days.


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Laemlak Y, Senawongse P, Saikaew P. Effect of 30-day toothbrushing on permeability of desensitizer occluded root dentin: In vitro study. M Dent J [Internet]. 2021 Jun. 25 [cited 2023 Nov. 30];41(Suppl):S19-S28. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/250716
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