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Objective: This study evaluated the dentin adhesion of a universal adhesive (Single Bond Universal; SBU) applied in either etch-and-rinse (E&R) or self-etch (SE) mode in the situations where 15 cm.H2O hydrostatic pulpal pressure (PP) was simulated during difference bonding and/or storage time.
Materials and Methods: Crown segments of 90 extracted third molars were used, in which direct communication between the PP simulation model and pulp chamber was created. They were divided into 2 groups according to application modes of SBU. Each group was divided into nine subgroups according to three bonded and stored condition (0/0 bonded and stored at 0 pressure, 0/15 bonded at 0, stored at 15 cm.H2O pressure, and 15/15 bonded and stored at 15 cm.H2O pressure) and three storage time (0 min-immediate measurement, 24 h and 3 months), then restored with resin composite. In all groups after storage in water, stick-shaped specimens from each tooth were prepared for micro-tensile bond strength (μTBS) test. Data were analyzed using Kruskal-Wallis and Mann-Whitney U tests (p<0.05).
Results: Regardless of the application mode and PP simulation, μTBS values at 24 h were the highest and those at 3 months were the lowest (p<0.05). Generally, the group SE15/15 presented lower μTBS than groups SE0/0. However, the group E&R15/15 showed lower μTBS than group E&R0/0, but not different from that group E&R0/15. Different modes of application revealed similar μTBS at immediate measurement, irrespective of PP simulation (p>0.05). But for longer storage periods, SBU with SE mode seemed to show higher or at least similar μTBS to that with E&R mode.
Conclusion: In conclusion, SBU bonded using SE mode provided similar or better dentin adhesion than that bonded using E&R mode. Simulation of PP during bonding procedure impaired the bond of SBU with SE mode, while PP simulation during storage seemed to have a negative impact on that with E&R mode.
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