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Objective: The aim of this study was to evaluate the antimicrobial potential of neem toothpaste against various oral microorganisms.
Materials and Methods: Toothpaste containing chloroform extract of neem leaf was prepared by TISTR, Ministry of Science and Technology, Thailand. Two commercial herbal toothpastes were selected for assessment of their antimicrobial activities compared to neem toothpaste. The toothpaste solution was prepared by adding 0.66 g of toothpaste to 1 mL of sterile deionizing water and mixed thoroughly. The suspensions of Streptococcus mutans KPSK 2, Lactobacillus casei ATCC 393, and Candida albicans ATCC 10231 were prepared and added to toothpaste solutions. Subsequent to 50 sec, the final solutions were brought to D/E neutralizing broth and cultured on Brain Heart Infusion agar, Rogosa agar and Sabouraud dextrose agar for the quantitation of S. mutans, L. casei and C. albicans, respectively at 37 °C for 24-48 h. A control was performed using sterile distilled water. The number of colonies was counted after incubation and expressed as colony forming unit (CFU)/ml. A statistical analysis was done by Kruskal-Wallis and Mann-Whitney tests.
Results: The result showed that all three herbal toothpastes had a significant suppressive effect against tested microorganisms compared with a control. Neem toothpaste was the most active against L. casei and C. albicans whereas other commercial toothpaste, tea tree oil toothpaste, was the most active against S. mutans and L. casei.
Conclusion: Information from the present study is scientific evidence to demonstrate that neem toothpaste could be selected as natural antimicrobial toothpaste for the prevention of caries-associated microorganisms. With appropriate tooth brushing, it is a simple way to gain good oral and dental health.
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