Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation

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Published: Jun 18, 2022
Keywords:
bone regeneration conditioned medium cell proliferation mesenchymal stem cell peptide

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Kornkamol Lunjanasatienchai
Resident in residency training program in orthodontics (International program), Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Niwat Anuwongnukroh
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Surachai Dechkunakorn
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Nathaphon Tangjit
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Sittiruk Roytrakul
Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand
Dumrongkiet Arthan
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
Jirattikarn Kaewmuangmoon
Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Hathaitip Sritanaudomchai
Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Abstract

Objectives: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) on human periodontal ligament stem cell (PDLSC) proliferation and osteogenic differentiation.


Materials and Methods: SHED-CM was collected after culturing for 48 h. The experimental SHED-CM groups were prepared by ultrafiltration together with diethyl ether precipitation to produce two different molecular weights fractions: D<3 and D3-10. PDLSCs were cultured with and without the SHED-CM fractions. To evaluate the effect of SHED-CM on PDLSCs proliferation and osteogenic differentiation, a CCK-8 assay and alizarin red S staining, respectively, was performed.


Results: SHED-CM-treated PDLSC demonstrated significantly increased cell proliferation from Days 4–8. In contrast, PDLSC calcified nodule formation was inhibited in the SHED-CM groups.


Conclusion: The results suggest that precipitated SHED-CM with less than 10 kDa molecular weight stimulated PDLSC proliferation and inhibited their osteogenic differentiation. These findings warrant further investigations to identify the peptides in SHED-CM to maximize PDLSC proliferation or osteogenic differentiation for future applications for bone regeneration.

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1.
Lunjanasatienchai K, Anuwongnukroh N, Dechkunakorn S, Tangjit N, Roytrakul S, Arthan D, Kaewmuangmoon J, Sritanaudomchai H. Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation. M Dent J [Internet]. 2022 Jun. 18 [cited 2024 Dec. 21];42(2):89-96. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/257012
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Vol. 42 No. 2 (2022): May-August 2022
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Original articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Chowchuen B, Thanaviratananich S, Chichareon V, Kamolnate A, Uewichitrapochana C, Godfrey K. A Multisite Study of Oral Clefts and Associated Abnormalities in Thailand: The Epidemiologic Data. Plast Reconstr Surg Glob Open. 2015; e583.

Myeroff C, Archdeacon M. Autogenous bone graft: donor sites and techniques. J Bone Joint Surg Am. 2011; 93: 2227-36.

Mazzetti MPV, Alonso N, Brock RS, Ayoub A, Massumoto SM, Eça LP. Importance of stem cell transplantation in cleft lip and palate surgical treatment protocol. J Craniofac Surg. 2018; 29: 1445-51.

Behnia H, Khojasteh A, Soleimani M, Tehranchi A, Atashi A. Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: a preliminary report. J Craniomaxillofac Surg. 2012; 40: 2-7.

Jitpibull J, Tangjit N, Dechkunakorn S, Anuwongnukroh N, Srikhirin T, Vongsetskul T, et al. Effect of surface chemistry-modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Eur J Oral Sci. 2021; 129: e12766.

Kunwong N, Tangjit N, Rattanapinyopituk K, Dechkunakorn S, Anuwongnukroh N, Arayapisit T, et al. Optimization of poly (lactic-co-glycolic acid)-bioactive glass composite scaffold for bone tissue engineering using stem cells from human exfoliated deciduous teeth. Arch Oral Biol. 2021; 123: 105041.

Nakajima K, Kunimatsu R, Ando K, Ando T, Hayashi Y, Kihara T, et al. Comparison of the bone regeneration ability between stem cells from human exfoliated deciduous teeth, human dental pulp stem cells and human bone marrow mesenchymal stem cells. Biophys. Res. Commun 2018; 497: 876-82.

Ide C, Nakai Y, Nakano N, Seo TB, Yamada Y, Endo K, et al. Bone marrow stromal cell transplantation for treatment of sub-acute spinal cord injury in the rat. Brain Res. 2010; 1332: 32-47.

Zimmermann CE, Gierloff M, Hedderich J, Acil Y, Wiltfang J, Terheyden H. Survival of transplanted rat bone marrow-derived osteogenic stem cells in vivo. Tissue Eng Part A. 2011; 17: 1147-56.

Ooi AT, Mah V, Nickerson DW, Gilbert JL, Ha VL, Hegab AE, et al. Presence of a putative tumor-initiating progenitor cell population predicts poor prognosis in smokers with non-small cell lung cancer. Cancer Res. 2010; 70: 6639-48.

Inoue T, Sugiyama M, Hattori H, Wakita H, Wakabayashi T, Ueda M. Stem cells from human exfoliated deciduous tooth-derived conditioned medium enhance recovery of focal cerebral ischemia in rats. Tissue Eng Part A. 2012; 19: 24-9.

Freshney RI. Basic principles of cell culture. Hobokan, NJ: John Wiley & Sons, Inc; 2006. p. 11-4.

Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, et al. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci. 2015; 35: 2452-64.

Yamagata M, Yamamoto A, Kako E, Kaneko N, Matsubara K, Sakai K, et al. Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice. J Stroke. 2013; 44: 551-4.

Sakai K, Yamamoto A, Matsubara K, Nakamura S, Naruse M, Yamagata M, et al. Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms. J Clin Invest. 2012; 122: 80-90.

Muhammad SA, Nordin N, Hussin P, Mehat MZ, Abu Kasim NH, Fakurazi S. Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes. PLoS One. 2020; 15: e0238449.

Luo P, Jiang C, Ji P, Wang M, Xu J. Exosomes of stem cells from human exfoliated deciduous teeth as an anti-inflammatory agent in temporomandibular joint chondrocytes via miR-100-5p/mTOR. Stem Cell Res Ther. 2019; 10: 216.

Wakayama H, Hashimoto N, Matsushita Y, Matsubara K, Yamamoto N, Hasegawa Y, et al. Factors secreted from dental pulp stem cells show multifaceted benefits for treating acute lung injury in mice. Cytotherapy. 2015; 17: 1119-29.

Matsushita Y, Ishigami M, Matsubara K, Kondo M, Wakayama H, Goto H, et al. Multifaceted therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for acute liver failure in rats. J Tissue Eng Regen Med. 2017; 11: 1888-96.

de Cara SPHM, Origassa CST, de Sá Silva F, Moreira MSN, de Almeida DC, Pedroni ACF, et al. Angiogenic properties of dental pulp stem cells conditioned medium on endothelial cells in vitro and in rodent orthotopic dental pulp regeneration. Heliyon. 2019; 5: e01560.

Hiraki T, Kunimatsu R, Nakajima K, Abe T, Yamada S, Rikitake K, et al. Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration. Oral Dis. 2020; 26: 381-90.

Omori M, Tsuchiya S, Hara K, Kuroda K, Hibi H, Okido M, et al. A new application of cell-free bone regeneration: immobilizing stem cells from human exfoliated deciduous teeth-conditioned medium onto titanium implants using atmospheric pressure plasma treatment. Stem Cell Res Ther. 2015; 6: 124.

Sugimura-Wakayama Y, Katagiri W, Osugi M, Kawai T, Ogata K, Sakaguchi K, et al. Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth. Stem Cells Dev. 2015; 24: 2687-99.

Izumoto-Akita T, Tsunekawa S, Yamamoto A, Uenishi E, Ishikawa K, Ogata H, et al. Secreted factors from dental pulp stem cells improve glucose intolerance in streptozotocin-induced diabetic mice by increasing pancreatic beta-cell function. BMJ Open Diabetes Res Care. 2015; 3: e000128.

Kichenbrand C, Velot E, Menu P, Moby V. Dental Pulp Stem Cell-Derived Conditioned Medium: An Attractive Alternative for Regenerative Therapy. Tissue Eng Part B Rev. 2019; 25: 78-88.

Marqus S, Pirogova E, Piva TJ. Evaluation of the use of therapeutic peptides for cancer treatment. J Biomed Sci. 2017; 24: 21.

Trubiani O, Pizzicannella J, Caputi S, Marchisio M, Mazzon E, Paganelli R, et al. Periodontal Ligament Stem Cells: Current Knowledge and Future Perspectives. Stem Cells Dev. 2019; 28: 995-1003.

Gonmanee T, Thonabulsombat C, Vongsavan K, Sritanaudomchai H. Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells. Arch Oral Biol. 2018; 88: 34-41.

Seo BM, Sonoyama W, Yamaza T, Coppe C, Kikuiri T, Akiyama K, et al. SHED repair critical-size calvarial defects in mice. Oral Dis. 2008; 14: 428-34.

Baglio SR, Pegtel DM, Baldini N. Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy. Front Physiol. 2012; 3: 359.

Maguire G. Stem cell therapy without the cells. Commun Integr Biol. 2013; 6: e26631.

Ali R, Rani R, Kumar S. New peptide based therapeutic approaches. Advances in protein chemistry. Jeddah: OMICS Group eBooks. 2013.

McGregor DP. Discovering and improving novel peptide therapeutics. Curr Opin Pharmacol. 2008; 8: 616-9.

Fosgerau K, Hoffmann T. Peptide therapeutics: current status and future directions. Drug Discov Today. 2015; 20: 122-8.

Kano F, Matsubara K, Ueda M, Hibi H, Yamamoto A. Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Synergistically Regenerate Transected Rat Peripheral Nerves by Altering Macrophage Polarity. Stem Cells. 2017; 35: 641-53.

Al Musaimi O, Jad YE, Kumar A, Collins JM, Basso A, Beatriz G, et al. Investigating green ethers for the precipitation of peptides after global deprotection in solid-phase peptide synthesis. Curr Opin Green Sustain Chem. 2018; 11: 99-103.

Mizushima T, Ohnishi S, Hosono H, Yamahara K, Tsuda M, Shimizu Y, et al. Oral administration of conditioned medium obtained from mesenchymal stem cell culture prevents subsequent stricture formation after esophageal submucosal dissection in pigs. Gastrointest Endosc. 2017; 86: 542-52.

Gul Amuk N, Kurt G, Karsli E, Ozcan S, Acar MB, Amuk M, et al. Effects of mesenchymal stem cell transfer on orthodontically induced root resorption and orthodontic tooth movement during orthodontic arch expansion protocols: an experimental study in rats. Eur J Orthod. 2020; 42: 305-16.