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Objectives: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) on human periodontal ligament stem cell (PDLSC) proliferation and osteogenic differentiation.
Materials and Methods: SHED-CM was collected after culturing for 48 h. The experimental SHED-CM groups were prepared by ultrafiltration together with diethyl ether precipitation to produce two different molecular weights fractions: D<3 and D3-10. PDLSCs were cultured with and without the SHED-CM fractions. To evaluate the effect of SHED-CM on PDLSCs proliferation and osteogenic differentiation, a CCK-8 assay and alizarin red S staining, respectively, was performed.
Results: SHED-CM-treated PDLSC demonstrated significantly increased cell proliferation from Days 4–8. In contrast, PDLSC calcified nodule formation was inhibited in the SHED-CM groups.
Conclusion: The results suggest that precipitated SHED-CM with less than 10 kDa molecular weight stimulated PDLSC proliferation and inhibited their osteogenic differentiation. These findings warrant further investigations to identify the peptides in SHED-CM to maximize PDLSC proliferation or osteogenic differentiation for future applications for bone regeneration.
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