Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation

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Published: Jun 18, 2022
Keywords:
bone regeneration conditioned medium cell proliferation mesenchymal stem cell peptide

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Kornkamol Lunjanasatienchai
Resident in residency training program in orthodontics (International program), Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Niwat Anuwongnukroh
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Surachai Dechkunakorn
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Nathaphon Tangjit
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Sittiruk Roytrakul
Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand
Dumrongkiet Arthan
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
Jirattikarn Kaewmuangmoon
Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Hathaitip Sritanaudomchai
Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Abstract

Objectives: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) on human periodontal ligament stem cell (PDLSC) proliferation and osteogenic differentiation.


Materials and Methods: SHED-CM was collected after culturing for 48 h. The experimental SHED-CM groups were prepared by ultrafiltration together with diethyl ether precipitation to produce two different molecular weights fractions: D<3 and D3-10. PDLSCs were cultured with and without the SHED-CM fractions. To evaluate the effect of SHED-CM on PDLSCs proliferation and osteogenic differentiation, a CCK-8 assay and alizarin red S staining, respectively, was performed.


Results: SHED-CM-treated PDLSC demonstrated significantly increased cell proliferation from Days 4–8. In contrast, PDLSC calcified nodule formation was inhibited in the SHED-CM groups.


Conclusion: The results suggest that precipitated SHED-CM with less than 10 kDa molecular weight stimulated PDLSC proliferation and inhibited their osteogenic differentiation. These findings warrant further investigations to identify the peptides in SHED-CM to maximize PDLSC proliferation or osteogenic differentiation for future applications for bone regeneration.

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1.
Lunjanasatienchai K, Anuwongnukroh N, Dechkunakorn S, Tangjit N, Roytrakul S, Arthan D, Kaewmuangmoon J, Sritanaudomchai H. Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation. M Dent J [Internet]. 2022 Jun. 18 [cited 2024 May 4];42(2):89-96. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/257012
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Vol. 42 No. 2 (2022): May-August 2022
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Original articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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