Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation

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Kornkamol Lunjanasatienchai
Niwat Anuwongnukroh
Surachai Dechkunakorn
Nathaphon Tangjit
Sittiruk Roytrakul
Dumrongkiet Arthan
Jirattikarn Kaewmuangmoon
Hathaitip Sritanaudomchai


Objectives: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) on human periodontal ligament stem cell (PDLSC) proliferation and osteogenic differentiation.

Materials and Methods: SHED-CM was collected after culturing for 48 h. The experimental SHED-CM groups were prepared by ultrafiltration together with diethyl ether precipitation to produce two different molecular weights fractions: D<3 and D3-10. PDLSCs were cultured with and without the SHED-CM fractions. To evaluate the effect of SHED-CM on PDLSCs proliferation and osteogenic differentiation, a CCK-8 assay and alizarin red S staining, respectively, was performed.

Results: SHED-CM-treated PDLSC demonstrated significantly increased cell proliferation from Days 4–8. In contrast, PDLSC calcified nodule formation was inhibited in the SHED-CM groups.

Conclusion: The results suggest that precipitated SHED-CM with less than 10 kDa molecular weight stimulated PDLSC proliferation and inhibited their osteogenic differentiation. These findings warrant further investigations to identify the peptides in SHED-CM to maximize PDLSC proliferation or osteogenic differentiation for future applications for bone regeneration.


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Lunjanasatienchai K, Anuwongnukroh N, Dechkunakorn S, Tangjit N, Roytrakul S, Arthan D, Kaewmuangmoon J, Sritanaudomchai H. Effect of human exfoliated deciduous teeth stem cell-derived conditioned medium on human periodontal ligament stem cell proliferation and osteogenic differentiation. M Dent J [Internet]. 2022 Jun. 18 [cited 2023 Dec. 6];42(2):89-96. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/257012
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