Effectiveness of different coated materials on root caries prevention under simulated tooth brushing: an in vitro study
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Abstract
Objective: Sealing tooth root surfaces with coating material is expected to represent an effective approach to preventing root caries. The aim of this study is to investigate the effect of tooth brushing on the efficacy of different coating materials in root caries prevention.
Materials and Methods: The experiment was conducted on root dentin specimens under simultaneous demineralization conditions. Fifty specimens were used in this study. The specimens were divided into 5 groups: Group 1 (control group), no treatment; Groups 2-3, Clinpro White Varnishapplication with or without brushing; and Groups 4-5, PRG Coat Barrierapplication with or without brushing. A multispecies biofilm model was formed on specimens that were exposed to 1% sucrose for 10 days. Then, the % cell viability, bacterial density, % surface microhardness loss, and lesion depth were assessed using a confocal laser scanning microscope, microcomputed tomography, and Knoop microhardness tester, respectively.
Results: The Kolmogorov–Smirnov test revealed a normal distribution of all data. Differences within and between mean values for groups were statistically analyzed using one-way ANOVA and Dunnett’s T3 post hoc test, respectively. No significant differences in bacterial density and biofilm thickness were found among the groups. A difference in the %surface microhardness loss was noted in the control group with relatively higher %cell viability compared with the other groups. The deepest carious lesion was found in the control group. The shallowest carious lesion was found in the PRG Coat Barrier-coated dentin-without brushing group. Brushing only increases the lesion depth on Clinpro White Varnish-coated dentin.
Conclusions: Applying either Clinpro White Varnish or PRG Coat Barrierhas the benefits of reducing the depth of root caries and increasing the hardness of demineralized root dentin. Brushing reduces the efficacy of Clinpro White Varnish.
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