Effect of double layer immediate dentin sealing technique on dentin permeability and microtensile bond strength
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Abstract
Objectives: To examine the effect of one-layer and two-layer Immediate dentin sealing (IDS) treatment on dentin permeability and microtensile bond strength (µTBS) of dentin and resin cement under simulated pulpal conditions.
Materials and Methods: Thirty extracted third molar teeth were cleaned, disinfected, and embedded in a resin block. The buccal surface was exposed to dentin, and the block was connected to a hydrostatic pressure device set at 20 cmH2O. The teeth were divided equally into three groups: NoIDS; no dentin treatment before direct cementation, 1IDS; a single layer of dental adhesive was applied, and 2IDS; a second layer was applied in addition to the first layer. Each group was further divided in half to perform the permeability test and µTBS test. For permeability test, the replica techniques captured droplets from the dentinal tubules, then investigated under a Scanning Electron Microscope. The size and total volume of droplets were measured and calculated. For µTBS test, 1IDS and 2IDS groups were contaminated with temporary cement, and then all groups were cemented with resin cement. The bonded samples were cut into small beams to conduct the µTBS test. Data analysis was performed using one-way ANOVA and Tukey's multiple comparisons. Additionally, the correlation between µTBS and total fluid volume was analyzed using Pearson correlation.
Results: The NoIDS group exhibited larger diameter droplets with irregular shapes, while the 1IDS group showed smaller droplets in higher numbers. No droplets were observed in the 2IDS group. In contrast, the bond strength was significantly higher in the 2IDS group, followed by the 1IDS group and the NoIDS group, respectively. There was a negative correlation between µTBS and total fluid volume.
Conclusion: Single-layer IDS technique reduced dentin permeability, whereas two-layer IDS effectively blocked dentin permeability, resulting in increased µTBS.
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