Mitochondrial dysfunction in the salivary glands of type 1 diabetes mellitus rats: effects of insulin and atorvastatin treatment

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Published: Oct 19, 2023
Keywords:
atorvastatin, insulin, mitochondria function, salivary gland, type 1 diabetes mellitus

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Benyanoot Kingkaew
Division of General Dentistry, Department of Family and Community Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand
Kawisara Wannasawet
Division of General Dentistry, Department of Family and Community Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand
Patchanee Chuveera
Division of General Dentistry, Department of Family and Community Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand
La-Ongdao Thongnak
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Anusorn Lungaphin
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Wasana Pratchayasakul
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand
Siriporn Chattipakorn
Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand Department of Oral Biology and Oral Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand
Jitjiroj Ittichaicharoen
Department of Oral Biology and Oral Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand

Abstract

Background and Objective: Type 1 Diabetes mellitus (T1DM) involves pancreatic beta-cell dysfunction and inadequate insulin production, leading to disrupted blood glucose levels and subsequent organ dysfunction. The aim of this study was to examine the influence of insulin and statins on oxidative stress and mitochondrial dysfunction in the salivary glands of streptozotocin-induced diabetic rats.


Material and Methods: Twenty male rats were divided into control (n=4) and experimental groups (n=16) of streptozotocin-induced diabetic rats. Diabetic rats were further divided into four groups (n=4/group) receiving vehicle, low-dose atorvastatin, low-dose insulin, or combined drugs for 4 weeks. Metabolic parameters were assessed via blood samples. Mitochondrial function in submandibular salivary glands was evaluated for reactive oxidative stress (ROS), mitochondrial membrane potential, and swelling analysis.


Results: Streptozotocin-induced T1DM rats displayed hyperglycemia, indicating heightened mitochondrial dysfunction in salivary glands. Mitochondrial ROS production and membrane depolarization were significantly elevated compared to normal rats (p<0.05). Atorvastatin, insulin, and combination therapy similarly mitigated mitochondrial dysfunction in induced T1DM rats (p<0.05). All three treatment groups significantly reduced plasma glucose, while combined therapy was the most effective.


Conclusion: Combined drug therapy demonstrated the highest efficacy in improving metabolic parameters. Atorvastatin, insulin, and combined therapy were equally effective in mitigating mitochondrial dysfunction in the salivary glands of induced T1DM rats. These findings suggest the potential of combination therapy for T1DM management. Further investigations are needed to understand their impact on salivary gland function and implications for oral health and overall well-being in individuals with T1DM.

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How to Cite
1.
Kingkaew B, Wannasawet K, Chuveera P, Thongnak L-O, Lungaphin A, Pratchayasakul W, Chattipakorn S, Ittichaicharoen J. Mitochondrial dysfunction in the salivary glands of type 1 diabetes mellitus rats: effects of insulin and atorvastatin treatment. M Dent J [Internet]. 2023 Oct. 19 [cited 2024 Dec. 2];43(suppl):S27-S36. Available from: https://he02.tci-thaijo.org/index.php/mdentjournal/article/view/264734
Issue
Vol. 43 No. suppl (2023): (October)
Section
Oral Presentation(MDRD2024)
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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