Primary contraction of harvested palatal sub-epithelial connective tissue grafts: a pilot study
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Abstract
Objective(s): To elucidate and compare the primary contraction of palatal sub-epithelial connective tissue grafts after harvesting at different times.
Materials and Methods: Ten patients who underwent soft tissue graft treatment, free gingival graft, or connective tissue graft. A 4-mm biopsy punch took each sample with 1 mm thickness after 1-mm-depth de-epithelialization. A total of 10 samples were recorded for graft contraction by a standardized photograph taken at 20(T1), 40(T2), 60(T3), 90(T4), 120 minutes(T5), and 24 hours(T6). Throughout the contraction investigation, the sample was incubated at 37 degrees Celsius, washed twice, and immersed in a new normal saline solution at 4 degrees Celsius between time points. The graft area was computed using ImageJ software. The graft contraction was calculated as the average percent of the original area (% of T1). Differences in graft contraction were analyzed using Friedman's Two-Way Analysis of Variance by Ranks and pairwise comparisons.
Results: Only one significant difference in graft contraction was found between T4 and T2, meaning that the graft area at 90 minutes decreased compared to the graft area at 40 minutes (p-value =0.047).
Conclusion: The harvested palatal sub-epithelial connective tissue graft size remained constant, except at 90 minutes after harvesting when it significantly decreased compared to 40 minutes. This study indicates that the sub-epithelial connective tissue graft can stay in normal saline solution outside the oral cavity with minimal contraction.
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