The Sensitivity of the Detection of Prostate Specific Antigen in Semen by ELISA

Main Article Content

Parimol Betset
Supawon Srettabunjong
Suvit Limawongpanee
Pattama Ekpo

Abstract

Background: In rape cases, examination of biological evidence, especially semen, is very important to identify whether the alleged crime actually happened, resulting in solving the case. Prostate-specific antigen (PSA) is an ideal forensic marker for semen identification, and enzyme-linked immunosorbent assay (ELISA) has been described for many years and found to be still one of the sensitive and efficient methods for PSA detection.


Objective: The purposes of this study were to optimize the assay conditions regarding reagents used and assay time and to determine its sensitivity for the detection of PSA in semen.


Materials and Methods: The semen sample were obtained from 20 consenting healthy Thai male volunteers aged 23-55 years during February – March 2011. All semen samples were stored at -80°C shortly after being secreted from the volunteers’ body and until being used for the analyses. The ELISA method performed in the study followed the previously reported protocol with some optimization. The sensitivity of ELISA in detecting PSA in semen was then studied.


Results: An optimal ELISA condition for the detection of PSA was described with consideration for reagents used and assay time. For sensitivity, the authors found that this method was easily capable of detecting PSA in semen diluted approximately 106 fold with the concentration of 0.56 ± 0.03 ng/ml.


Conclusion: The optimal conditions of an ELISA method for the detection of PSA in semen described was found to be one of the sensitive and efficient methods for the detection of PSA in semen for routine analysis n forensic laboratories, especially in case of a lot of the evidence examined daily, and for further research analysis.

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1.
Betset P, Srettabunjong S, Limawongpanee S, Ekpo P. The Sensitivity of the Detection of Prostate Specific Antigen in Semen by ELISA. Rama Med J [Internet]. 2012 Jun. 29 [cited 2024 Mar. 29];35(2):70-8. Available from: https://he02.tci-thaijo.org/index.php/ramajournal/article/view/117894
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Original Articles

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