A Comparision of the Cryodamage on Human Sperm in Vapor and Slow Freezing
Keywords:
Sperm cryopreservation, Vapor phase liquid nitrogen, Slow freezingAbstract
Objective: To evaluate the percentage of motile, vitality, morphology, and DNA integrity change on human sperm after freeze-thawing compared between vapor and slow freezing method.
Setting: The Infertility and ART Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital.
Materials and Methods: In this experimental study, forty-five normozoospermic volunteer donors were used in the study. After swim up of the sperm, the sperm suspensions were divided into two equal parts, one part was kept as a control (frozen with vapor phase liquid nitrogen) and the other was used for the frozen with slow freezing. After one month of cryostorage, the sperm samples were thawed. The percentage of motile, vitality, morphology, and DNA integrity change were determined. The data was analyzed by a paired t-test.
Results: The percentage of the sperm motility with slow freezing was observed to decrease from 85.9 to 45.5 while that of the control declined to 40.7. The percentage of motile change of human sperm was significantly better following freezing with slow freezing than the control. The percentage of vitality change on human sperm assessed with slow freezing was significantly higher than in the vapor phase liquid nitrogen method. No significant differences in post thaw normal morphologically sperm and DNA integrity were compared between the vapor and slow freezing method.
Conclusion: The percentage of motile and vitality change of human sperm with slow freezing is superior to that of the vapor phase liquid nitrogen method.
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