Renal Transforming Growth Factor Beta Gene Expression

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Prapaipit Srimawong
Anchalee Tantiwetrueangdet
Koset Pinpradap
Vasant Sumethkul
Vachira Kochakarn
Kittinut Kijvikai
Krisada Ratana-Olarn
Suchart Chaimuangraj
Charoen Leenanupunth
Sopon Jirasiritam
Wisoot Kongchareonsombat
Chagriya Kitiyakara

Abstract

Background: Fibrosis in the renal biopsy is associated with poor long term outcome in many kidney diseases. However, fibrosis occurs at a late stage when the kidney is irreversibly damaged. Molecular biology techniques are currently being investigated to identify early prognostic markers in kidney diseases. Reverse transcriptase real-time PCR (RT-qPCR) is a highly sensitive technique capable of detecting small changes in gene expression, Transforming growth factor-gif.latex?\dpi{100}&space;\beta1 (TGF-gif.latex?\dpi{100}&space;\beta1) is a key mediator of fibrosis and is expected to be increased in damaged tissues. This is a pilot study to investigate the feasibility of the using RT-qPCR to study gene expression of TGF-31 in human kidney tissues.


Methods: RNA was extracted from normal and diseased human kidneys with fibrosis and reverse transcribed. TGF-gif.latex?\dpi{100}&space;\beta1 gene expression was studied by multiplex RT-qPCR using cyclophilin A as a housekeeping gene. Relative gene expression was calculated from 2-DDCT method.


Results: The expression TGF-gif.latex?\dpi{100}&space;\beta1 in three different areas of the same kidney were similar The expression of TGF-bgif.latex?\dpi{100}&space;\beta1 was 4-5 fold higher in disease kidney tissues compared to normal (p < 0.001).


Summary: TGF-P1 gene expression can be measured from human kidney using RT-qPCR. There are minimal effects of tissue sampling on gene expression levels, hence tissue obtained from a kidney biopsy should be representative of the whole kidney cortex. The expression of TGF-gif.latex?\dpi{100}&space;\beta1 is higher in fibrotic kidneys. Future studies are necessary to determine if the TGF-gif.latex?\dpi{100}&space;\beta1 gene expression markers can predict disease progression.

Article Details

How to Cite
Srimawong, P., Tantiwetrueangdet, A., Pinpradap, K., Sumethkul, V., Kochakarn, V., Kijvikai, K., Ratana-Olarn, K., Chaimuangraj, S., Leenanupunth, C., Jirasiritam, S., Kongchareonsombat, W., & Kitiyakara, C. (2009). Renal Transforming Growth Factor Beta Gene Expression. Ramathibodi Medical Journal, 32(1), 3–12. Retrieved from https://he02.tci-thaijo.org/index.php/ramajournal/article/view/153226
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Original Articles

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