Commercial DNA Typing Test Kit Comparison for Multipurpose of Human Identification

Jittima Shotivaranon; Budsaba  Rerkamnuaychoke

doi:10.33165/rmj.2022.45.1.253355

Authors

Jittima Shotivaranon Department of Pathology, Faculty of Medicine, Ramathibodi hospital, Mahidol University, Bangkok, Thailand
Budsaba Rerkamnuaychoke Department of Pathology, Faculty of Medicine, Ramathibodi hospital, Mahidol University, Bangkok, Thailand

DOI:

https://doi.org/10.33165/rmj.2022.45.1.253355

Keywords:

DNA fingerprint, Human identification, STR profile

Abstract

Human identification is applied to prove interpersonal relationships by using commercial test kits of a single tube multiplex PCR on DNA repeated sequence (short tandem repeat, STR) genetic markers. Each commercial test kit has different limitation and advantage. The objective of this report is to compare the advantage and limitation of 8 commercial test kits (GlobalFiler Express, GlobalFiler™ IQC / GlobalFiler, Verifiler Express, Verifiler Plus, Investigator 24 Plex QS, Investigator 24 Plex Go, Investigator 26 Plex QS, and PowerPlex Fusion6C). We not only reviewed and compared the strengths and limitations of these 8 commercial test kits but also compared DNA profiles generated by 3 commercial test kits which were used in routine paternity service of Human Genetic Laboratory, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University. The data demonstrated that combined results of different commercial test kits was able to solve the interpretation of mutation or exclusion cases in some situations.

Author Biography

Jittima Shotivaranon, Department of Pathology, Faculty of Medicine, Ramathibodi hospital, Mahidol University, Bangkok, Thailand

Department of Pathology, Faculty of Medicine Ramathibodi Hospital

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