Commercial DNA Typing Test Kit Comparison for Multipurpose of Human Identification
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Abstract
Human identification is applied to prove interpersonal relationships by using commercial test kits of a single tube multiplex PCR on DNA repeated sequence (short tandem repeat, STR) genetic markers. Each commercial test kit has different limitation and advantage. The objective of this report is to compare the advantage and limitation of 8 commercial test kits (GlobalFiler Express, GlobalFiler™ IQC / GlobalFiler, Verifiler Express, Verifiler Plus, Investigator 24 Plex QS, Investigator 24 Plex Go, Investigator 26 Plex QS, and PowerPlex Fusion6C). We not only reviewed and compared the strengths and limitations of these 8 commercial test kits but also compared DNA profiles generated by 3 commercial test kits which were used in routine paternity service of Human Genetic Laboratory, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University. The data demonstrated that combined results of different commercial test kits was able to solve the interpretation of mutation or exclusion cases in some situations.
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References
Jignal P, Shaikh MG, Darshan M. Forensic conception: DNA typing of FTA spotted samples. J Appl Biol Biotech. 2014;2(4):021-029. doi:10.7324/JABB.2014.2404
Scientific Working Group on DNA Analysis Methods (SWGDAM). SWGDAM Interpretation Guidelines for Autosomal STR Typing by Forensic DNA Testing Laboratories. SWGDAM; 2017. Updated July 13, 2021. Accessed March 9, 2022. https://1ecb9588-ea6f-4feb-971a-73265dbf079c.filesusr.com/ugd/4344b0_3f94c9a6286048c3924c58e2c230e74e.pdf
Butler JM. Advanced Topics in Forensic DNA Typing: Interpretation. Academic Press; 2014.
Butler JM. Fundamentals of Forensic DNA Typing. Academic Press/ Elsevier; 2010.
Butler JM. Forensic DNA Typing: Biology, Technology, and Genetics of STR Markers. 2nd ed. Elsevier Academic Press; 2005.
Rerkamnuaychoke B. Recent Advances in Forensic Genetics. Faculty of Medicine Ramathibodi Hospital, Mahidol University; 2018.
Boonderm N, Suriyanratakorn D, Sangpueng S, Onthong N, Nettakul A, Waiyawuth W. Population genetic data of 21 STR markers in Thais of southern border provinces of Thailand. Forensic Sci Int Genet Suppl Ser. 2017;6:e523-e525. doi:10.1016/j.fsigss.2017.09.205
Promega Corporation. PowerPlex® Fusion 6C System for Use on the Applied Biosystems® Genetic Analyzers, Technical Manual, 2018. Accessed March 9, 2022. https://worldwide.promega.com/-/media/files/resources/protocols/technical-manuals/101/powerplex-fusion-6c-system-protocol.pdf?rev=a8d2566e44ca493db6da4c0256baee53&sc_lang=en
QIAGEN®. Investigator® 26plex QS Handbook, 2019. Accessed March 9, 2022. https://dev04.qiagen.com/gb/resources/download.aspx?id=88978064-d8b6-4c33-8350-418d90ef42bb&lang=en
National Institute of Standards and Technology. Commercially Available STR Multiplex Kits. Updated December 31, 2009. Accessed March 9, 2022. https://strbase.nist.gov/str_D8S1179.htm
Buckleton JS, Bright JA, Taylor D, eds. Forensic DNA Evidence Interpretation. 2nd ed. CRC Press; 2018.
Shotivaranon J, Chirachariyavej T, Leetrakool N, Rerkamnuaychoke B. DNA database of populations from different parts in the Kingdom of Thailand. Forensic Sci Int Genet. 2009;4(1):e37-e38. doi:10.1016/j.fsigen.2009.02.009
Dawid AP, Mortera J, Pascali VL. Non-fatherhood or mutation? a probabilistic approach to parental exclusion in paternity testing. Forensic Sci Int. 2001;124(1):55-61. doi:10.1016/s0379-0738(01)00564-3
Ayres KL, Balding DJ. Paternity index calculations when some individuals share common ancestry. Forensic Sci Int. 2005;151(1):101-103. doi:10.1016/j.forsciint.2004.10.007
Gjertson DW, Brenner CH, Baur MP, et al. ISFG: recommendations on biostatistics in paternity testing. Forensic Sci Int Genet. 2007;1(3-4):223-231. doi:10.1016/j.fsigen.2007.06.006
Liu Y, Liu Y, Guo J, et al. Genetic polymorphism of 29 STR loci in the Hunan Han population from China. Forensic Sci Res. 2017;4(4):351-353. doi:10.1080/20961790.2017.1306430