Postmenopausal Osteoporosis in Relation to Toll-like Receptor-7 and HLA DQB1 Gene Expression on Peripheral Blood Mononuclear Cells

Authors

  • Wanwisa Waiyaput Office of Academic Affairs, Research, and Innovation, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
  • Mayuree Jirapinyo Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
  • Charnchai Suchartwattanachai Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
  • Boonsong Ongphiphadhanakul Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
  • Somphoch Pumipichet Department of Obstetrics and Gynecology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand
  • Areepan Sophonsritsuk Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

Keywords:

Postmenopause, Osteoporosis, Women, Toll-like receptor-7, Expression

Abstract

Background: Data from genome wide association study have demonstrated that the toll-like receptor-7 gene (TLR7), a well-known role in innate immunity, may participate in the development of postmenopausal osteoporosis. The HLA DQB1 gene is part of human leukocyte antigen (HLA) complex. HLA DQB1 plays a critical role in the immune system.

Objective: To determine the association of postmenopausal osteoporosis and transcripts of TLR7 and  HLA DQB1 from peripheral blood mononuclear cells (PBMCs). 

Methods: The study groups included 25 postmenopausal women with normal bone mineral density (BMD) and the other 25 women with osteoporosis. Blood was drawn from the subjects for bone markers and PBMCs preparation. RNA was then isolated and quantitative RT-PCR was performed for TLR7 and  HLA DQB1 genes.

Results: PBMCs expression of both TLR7 and HLA DQB1 were not significantly different between postmenopausal women with normal BMD and those with osteoporosis. (P = 0.567 and P = 0.248, respectively).  

Conclusions: It is unlikely that TLR7 and HLA DQB1 contribute a major role on postmenopausal osteoporosis in Thai women.

 

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1.
Waiyaput W, Jirapinyo M, Suchartwattanachai C, Ongphiphadhanakul B, Pumipichet S, Sophonsritsuk A. Postmenopausal Osteoporosis in Relation to Toll-like Receptor-7 and HLA DQB1 Gene Expression on Peripheral Blood Mononuclear Cells. Rama Med J [Internet]. 2016 Mar. 29 [cited 2024 Dec. 22];39(1):45-54. Available from: https://he02.tci-thaijo.org/index.php/ramajournal/article/view/57596

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Vol. 39 No. 1 (2016): January-March

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Original Articles

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