Effect of In vitro Culture of Human Myometrial and Fibroid Smooth Muscle Cells on Protein Estrogen Receptor
Keywords:
Leiomyoma, fibroid, myometrium, estrogen receptor, in vitro cultureAbstract
Background: Uterine leiomyomas (fibroids, myomas) are the most common benign smooth muscle cell tumors in the reproductive tract. The sex hormone estrogen has been implicated as a major regulator of fibroid growth. Estrogen exerts its physiological effects on the target cells by binding to specific nuclear receptors, the estrogen receptor α (ERα) and estrogen receptor ß (ERß).
Objective: To assess the expression of ERα and progesterone receptor (PR) on primary culture smooth muscle cells (SMCs) from myometrial and fibroid tissue for the potential use of primary culture SMCs as an experimental model for studying ER activity, PR activity, and drug testing.
Methods: Quantitative real-time PCR (qPCR) and Western blot analysis were used to compare ERα between paired myometrial and fibroid tissues, as well as cells isolated from the same tissues and cultured up to the three passages.
Results: ERα and PR levels in cultured cells were rapidly lost comparing with whole tissues for both myometrium and fibroid. Moreover, ERα protein was degraded after tissue digestion prior to the culturing process.
Conclusions: Primary human myometrial and fibroid smooth muscle cell cultures are not suitable for studying ER in vitro model. The stable ER and PR cell lines were the model of choice.
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Copyright (c) 2016 By the authors. Licensee RMJ, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
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