การศึกษาประสิทธิภาพของการตรวจหาสารพันธุกรรมของเชื้อไวรัสโคโรนา 2019 แบบขั้นตอนเดียว เปรียบเทียบกับการตรวจหาสารพันธุกรรมของเชื้อไวรัสโคโรนา 2019 แบบ Real time RT-PCR
Main Article Content
Abstract
Objective: The COVID-19 pandemic has been caused by SARS-CoV-2 infections. RTA-AFRIMS provided real-time reverse transcription polymerase chain reaction (real-time RT-PCR), one of the gold standards for SARS-CoV-2 detection, in response to the pandemic and support of its military missions and the Thai public health. Cobas Liat SARS-CoV-2, which was the first U.S. Food and Drug Administration authorized Point-of-Care RT-PCR, claimed to have highly sensitive and rapid SARS-CoV-2 nucleic acid detection. Considering establishing this new diagnostic technology in the RTA-laboratory, we evaluated its efficiency, sensitivity, and specificity.
Material and Methods: Sixty nasopharyngeal swabs in viral transport medium were tested by real-time RT-PCR and the Cobas Liat SARS-CoV-2 system. The results from the two procedures were then analyzed for sensitivity and specificity.
Results: The results of the two assays showed 100% agreement, both positive and negative, at a correlation level of 0.98.
Conclusion: Point-of-Care Cobas Liat SARS-CoV-2 system produced results concordant with real-time RT-PCR and required less time and processing. Based on this assessment, the system was established in the diagnostic laboratory to support in military and civilian tasks.
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