Development of military product: Hemostatic gel from Chromolaena odorata leaf extract
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Abstract
Objective: To develop a hemostatic gel from Chromolaena odorata leaf extract with the ability to stop bleeding for military application. Materials and Methods: 70% Ethanolic C. odorata leaf extract and its hemostatic gel were quality controlled by analysis with High Performance Liquid Chromatography (HPLC) to determine the amount of Scutellarein tetramethyl ether. The formulas of hemostatic gel were developed and selected to produce on a pilot scale. Results: The method was validated for its specificity, linearity (range 6.25 - 200 µg/ml with correlation coefficient (r) 0.9998), accuracy (recovery 101.12 ± 3.78%), and precision (%RSD 3.74). The selected formulation contained 5% w/w of C. odorata leaf extract with 70% ethanol and propylene glycol as solvents in the formula. Carbopol® 940 2% w/w was used as a gelling agent. The final product was a greenish-brown gel with a pH value of 4.16, a viscosity of 626 cP, and scutellarein tetramethyl ether content of 68.95 µg/ml, which fall within the effective concentration range for hemostatic activity. Conclusions: The obtained prototype product, packaged in 100-gram white plastic tubes, passed quality assessment to show the amount of hemostatic active component to be within its effective concentration range. It has been delivered to test users, including the Royal Thai Army Medical Department, Medical coordination center Southern Border Provinces (CDC), Narathiwat Task Force, Pattani Task Force, Yala Task Force, and Songkhla Task Force to be used for further testing.
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