Antioxidant and Antiproliferative Activities against HepG2 Cell Line of Ethanolic Extract from Leaves and Shoots of Spilanthes acmella

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Anantasak Piraksa
Chananyou Seeka
Netnarin Panseetum
Sukanya Pattha
Piyapong Choomsri
Orathai Panpetch
Bunliang Suphim


The purpose of this study was to investigate the total phenolic contents, antioxidant activity, and antiproliferative activity against HepG2 cell line from leaves and shoots of Spilanthes acmella. Total phenolic content was analyzed by Folin-Ciocalte method. Antioxidant activity was used DPPH radical scavenging assay and FRAP (Ferric reducing/antioxidant potential). The antiproliferative activity against liver cancer (HepG2) cell line was determined by sulforhodamine B (SRB) assay. The result showed that the ethanolic extract from leaves at the Paddy field had the highest amount of total phenolic content. There was 14.32±0.01 μg GAE/mg sample. Antioxidation activity assay, the result showed that the leaves extract at Paddy field had the highest antioxidant activity which showed IC50 value of DPPH radical at 262.21±1.13 μg/mL and FRAP value of 252.71±2.33 μg TEAC/mg sample. Antiproliferative activity against liver cancer (HepG2) cell line, the result showed that the leaves extract at the Corn field had the highest antiproliferative activity which showed IC50 value of growth inhibition at 464.70±133.57 μg/mL. The result show that the antioxidant activity of leaves and shoots extract at Corn field and Paddy field had the statistical significantly positive correlation with HepG2 cell growth inhibition at 0.05 (R2 = 0.8727, p < 0.05). These results may be used as preliminary data for further study of the anticancer activity against liver cancer cells of Spilanthes acmella extract.

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Piraksa A, Seeka C, Panseetum N, Pattha S, Choomsri P, Panpetch O, Suphim B. Antioxidant and Antiproliferative Activities against HepG2 Cell Line of Ethanolic Extract from Leaves and Shoots of Spilanthes acmella. Health Sci J Thai [Internet]. 2022 Jan. 25 [cited 2024 Apr. 12];4(1):33-4. Available from:
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