Antioxidant and Anti-inflammatory Activities of Arundina graminifolia Leaf Extract


  • Wanisa Punfa School of Traditional and Alternative Medicine, Chiang Rai Rajabhat University, Chiang Rai, 57100, Thailand
  • Chakkrit Khanaree School of Traditional and Alternative Medicine, Chiang Rai Rajabhat University, Chiang Rai, 57100, Thailand
  • Payungsak Tantipaiboonwong School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
  • Kanjana Pangjit School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
  • Piyawan Nuntaboon School of Medical Sciences, University of Phayao, Phayao 56000, Thailand
  • Pilaiporn Thippraphan College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand
  • Komsak Pintha Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand



Anti-inflammatory, Antioxidant, Arundina graminifolia, Free radicals


Arundina graminifolia (D.Don) Hochr. has been used as a traditional medicine for detoxification, anti-arthritis, and antidote for many years. This study aimed to determine the biological property of Arundina graminifolia leaf extract (ALE) for antioxidant and anti-inflammatory activities. Arundina graminifolia leaves were extracted with 70% ethanol, and antioxidant activity was evaluated by DPPH and ABTS assay. The iron-binding capacity of ALE was measured by spectrophotometric technique. Lipid peroxidation assay was determined using the thiocyanate method. Moreover, the production of TNF-α and IL-6 was measured by an ELISA kit. Our results showed that ALE containing total phenolic and flavonoid contents exhibited antioxidant activity by scavenging free radicals. The IC50 values of ALE by DPPH and ABTS radical scavenging assay were 155.17±3.61 and 13.82±0.38 µg/mL, respectively. Furthermore, ALE could inhibit free radical production via the Fenton reaction by complex formation with Fe3+-NTA. The scavenging activity of ALE in hemoglobin-induced lipid peroxidation resulted in 42% inhibition. Besides, ALE significantly inhibited LPS-induced pro-inflammatory mediators, including the production of NO and IL-6. These findings suggest that ALE could be used as a functional food or pharmaceutical product containing antioxidant and anti-inflammatory activities and providing therapeutic potential for treating inflammatory diseases.


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How to Cite

Punfa W, Khanaree C, Tantipaiboonwong P, Pangjit K, Nuntaboon P, Thippraphan P, Pintha K. Antioxidant and Anti-inflammatory Activities of Arundina graminifolia Leaf Extract. Health Sci J Thai [Internet]. 2023 Jun. 23 [cited 2023 Dec. 2];5(3):9-17. Available from:



Original articles