Effect of SEffect of Spray from Herb Extract on Inhibition of Bacteria in Public Toilet m Herb Extract on Inhibition of Bacteria in Public Toilet


  • Rotruedee Chotigawin Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Kowit Suwannahong Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Kanyarat Promnoi Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Fahruthai Kongkhuntod Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Warinton Arpornhirun Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Natthanicha Phumanee Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand
  • Taddao Pahasup-anan Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand




Herb extract, Clove, Ginger, Public toilet, Antibacterial spray


This research aimed to test the efficacy of herbal extract sprays in inhibiting bacteria on surfaces in public toilets. The samples were collected from the surfaces of toilet seats, door knobs or locks, and sink taps in two public toilets located on Bangsaen Beach, Chonburi province, using swab techniques. Ginger, clove, and a mixture of ginger and clove extracts were produced through the maceration extraction method in absolute ethanol for seven days, and their antimicrobial activity was tested using agar diffusion methods. The chosen herb extract was then tested for its ability to inhibit surface bacteria by spraying it onto a tile that represents a surface material in the toilet. The results showed that bacteria contamination was found on toilet seats and door locks in both toilets. The clove extract demonstrated the highest antimicrobial activity against the sampled bacteria, followed by the mixture of ginger and clove extract, and the ginger extract. When used as a surface disinfectant spray, the clove extract achieved more than 99% reduction of bacteria on the sample surface when using 5 ml, which is equivalent to the efficacy of a conventional chemical antibacterial spray. This study suggests that clove extract could be used as a bioactive substance in antibacterial sprays to inhibit surface bacteria and improve toilet sanitation.


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Abney SE, Bright KR, McKinney J, Ijaz MK, Gerba CP. Toilet Hygiene-Review and Research Needs. Journal of Applied Microbiology 2021; 131(6): 2705-2714. https://doi.org/10.1111/jam.15121

Chitpirom K, Kaigate B, Singhakajen V. Hygienic condition and Satisfaction of Toilet Users and Contamination of Coliform Bacteria in Public Toilet inside the Supermarkets, Bangkok. The Public Health Journal of Burapha University 2014; 9(1): 141-151. (In Thai)

Boonkhao L, Namwong N.Standard Appraisal of Toilets and Assessment of Coliform Bacteria in the Public Toilets of Ubon Ratchathani University. Journal of Science and Technology, Ubon Ratchathani University 2018; 21(3): 140-151. (In Thai)

Aithinne, KAN, Cooper CW, Lynch RA, Johnson A. Toilet plume aerosol generation rate and environmental contamination following bowl water inoculation with Clostridium difficile spores. American Journal of Infection Control 2019; 47(5): 515-520. https://doi.org/10.1016/j.ajic.2018.11.009

Mkrtchyan HV, Russell CA, Wang N, Cutler RR. Could Public Restrooms Be an Environment for Bacterial Resistomes? PLOS ONE 2013; 8(1): e54223. https://doi.org/10.1371/journal.pone.0054223

Kanayama KA, Takahashi H, Yoshizawa S, Tateda K, Nakanishi Y, Kaneko A, Kobayashi I. Public Health and Healthcare-Associated Risk of Electric, Warm-water Bidet Toilets. Journal of Hospital Infection 2017; 97(3): 296-300. https://doi.org/10.1016/j.jhin.2017.07.021

Rattanasinganchan P. The Hazard of Corrosive Substances in Housecleaning Products. HCU Journal of Health Science 2013; 16(32): 157-168. (In Thai)

Department of Disease Control. The Department of Disease Control Warns Farmers That "Dangerous Toilet Cleaners" Should Not Spray in Rice Paddy. [Internet]. 2022 [Cited 3 March 2023]. Available from https://ddc.moph.go.th/brc/news.php?news=26753&deptcode=brc.

Nooheet W. Inhibition of Pathogenic Bacteria Contaminating Exposed Surfaces by Extract of Zingiberaceae Family. [Thesis]. Prince of Songkla University; 2013. (In Thai)

Kiatsomboon N. Properties and Application of Antimicrobial Films Incorporated with Encapsulated Clove Extract. [Thesis]. Prince of Songkla University; 2009. (In Thai)

Neelapong W, Phonyotin B, Sittikijyothin W. Extraction of Active Compounds from Thai Herbs: Powder and Extract. The Journal of KMUTNB 2019; 29(1): 157-166. (In Thai) https://doi.org/10.14416/j.kmutnb.2018.12.10

Department of Health. National Standards for Public Toilets. Bureau of Environmental Health, Ministry of Public Health 2012. (In Thai)

Siripraphakachonkit D, Thanaboripat D, Laosinwattana J. Efficacy of Plant Extracts from Chinese Rice Flower and Clove on Growth Inhibition and Aflatoxin Production of Aspergillus flavus IMI 242684 and Aspergillus parasiticus IMI 102566in Maize. Journal of Science Ladkrabang 2015; 24(1): 71-88. (In Thai)

Tunvongvinis T, Chanchaem W. Investigation for the Antibacterial Activity of Five Citrus Fruits' Peel Extracts. Ramkhamhaeng Research Journal of Sciences and Technology 2021; 24(1): 27-33.

Meesilp N. Growth and Biofilm Generation of Bacteria Strain Escherichia Coli on Stainless Steel Surface by Cultivation with Milk. UTK Research Journal 2020; 14(1): 145-154. (In Thai)

Uacharoen K, Taothong W, Kumvilanon J, Chansri R, Keatpornpitak S, Seedaya P. Antibacterial Activity of Globba Winitii Rhizome Extracts Against Staphylococcus aureus. PTU Journal of Science and Technology 2020; 1(2): 34-44. (In Thai)

Vardoulakis S, Espinoza ODA, Donner E. Transmission of COVID-19 and Other Infectious Diseases in Public Washrooms: A Systematic Review. Science of the Total Environment 2022; 803: 149932. https://doi.org/10.1016/j.scitotenv.2021.149932

Best E, Parnell P, Couturier J, Barbut F, Le Bozec A, Arnoldo L, Madia A, Brusaferro S, Wilcox MH. Environmental Contamination by Bacteria in Hospital Washrooms According to Hand-Drying Method: A Multi-Centre Study. Journal of Hospital Infection 2018; 100(4): 469-475. https://doi.org/10.1016/j.jhin.2018.07.002

Thaisuchat H, Boonkorn P. In vitro Control of Fungal Contamination in Stored Garlic by Herbal Extracts and Microbial Antagonists. KKU Science Journal 2014; 42(4): 771-780. (In Thai)

Parham S, Kharazi AZ, Bakhsheshi-Rad HR, Nur H, Ismail AF, Sharif S, Ramakrishna S, Berto F. Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials. Antioxidants 2020; 9(12): 1-36. https://doi.org/10.3390/antiox9121309

Lekjing S, Chinarak K. Effect of Clove Essential Oil on Film Properties, Antioxidant and Antimicrobial Activities of Chitosan Film. KKU Research Journal (Graduate Studies) 2019; 19(4): 172-189. (In Thai)

Zambak Ö, Özkal A, Özkal SG. Production of Clove Extract Loaded Pullulan and Whey Protein Nanofibers as Antioxidant and Antibacterial Agent. Journal of Applied Polymer Science 2022; 139(46): 1-15. https://doi.org/10.1002/app.53141

Felgueiras HP, Homem NC, Teixeira MA, Ribeiro ARM, Antunes JC, Amorim MTP. Physical, Thermal, and Antibacterial Effects of Active Essential Oils with Potential for Biomedical Applications Loaded onto Cellulose Acetate/Polycaprolactone Wet-Spun Microfibers. Biomolecules 2020; 8(10): 1-20. https://doi.org/10.3390/biom10081129

Haasbroek K, Yagi M, Yonei Yoshikazu. Staphylococcus aureus Biofilm Inhibiting Activity of Advanced Glycation Endproduct Crosslink Breaking and Glycation Inhibiting Compounds. Antibiotics 2022; 11(10): 1-13. https://doi.org/10.3390/antibiotics11101412

Ali R, Khamis T, Enan G, El-Didamony G, Sitohy B, Abdel-Fattah G. The Healing Capability of Clove Flower Extract (CFE) in Streptozotocin-Induced (STZ-Induced) Diabetic Rat Wounds Infected with Multidrug Resistant Bacteria. Molecules 2022; 27(7): 1-23. https://doi.org/10.3390/molecules27072270

Elnaggar WA, Taha TH, El-Deeb NM, Arafat HH. Efficacy of Non-Cytotoxic Doses of Some Medicinal Plant Extracts as Antibacterial and Anti-biofilm Agents Against Cariogenic Bacterium Streptococcus mutans. Biosciences Biotechnology Research Asia 2016; 13(2): 1279-1284. https://doi.org/10.13005/bbra/2163




How to Cite

Chotigawin R, Suwannahong K, Promnoi K, Kongkhuntod F, Arpornhirun W, Phumanee N, Pahasup-anan T. Effect of SEffect of Spray from Herb Extract on Inhibition of Bacteria in Public Toilet m Herb Extract on Inhibition of Bacteria in Public Toilet. Health Sci J Thai [Internet]. 2023 Sep. 7 [cited 2023 Dec. 8];5(4):9-16. Available from: https://he02.tci-thaijo.org/index.php/HSJT/article/view/261722



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