Effect of SEffect of Spray from Herb Extract on Inhibition of Bacteria in Public Toilet m Herb Extract on Inhibition of Bacteria in Public Toilet

Abstract

This research aimed to test the efficacy of herbal extract sprays in inhibiting bacteria on surfaces in public toilets. The samples were collected from the surfaces of toilet seats, door knobs or locks, and sink taps in two public toilets located on Bangsaen Beach, Chonburi province, using swab techniques. Ginger, clove, and a mixture of ginger and clove extracts were produced through the maceration extraction method in absolute ethanol for seven days, and their antimicrobial activity was tested using agar diffusion methods. The chosen herb extract was then tested for its ability to inhibit surface bacteria by spraying it onto a tile that represents a surface material in the toilet. The results showed that bacteria contamination was found on toilet seats and door locks in both toilets. The clove extract demonstrated the highest antimicrobial activity against the sampled bacteria, followed by the mixture of ginger and clove extract, and the ginger extract. When used as a surface disinfectant spray, the clove extract achieved more than 99% reduction of bacteria on the sample surface when using 5 ml, which is equivalent to the efficacy of a conventional chemical antibacterial spray. This study suggests that clove extract could be used as a bioactive substance in antibacterial sprays to inhibit surface bacteria and improve toilet sanitation.