Development of Paper-Based Device for Cholinesterase Enzyme Analysis from Exposure to Organophosphate Pesticides
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Abstract
This research study aimed to develop a paper-based device for cholinesterase enzyme analysis from exposure to organophosphate pesticides using the chemical reaction between 5,5-dithiobis-2-nitrobenzoic acid (DTNB), acetylthiocholine chloride, and cholinesterase enzyme caused a color change from colorless to yellow on the test paper. Quantitative analysis was done by taking pictures with a mobile phone (iPhone 12). Images were processed with an image processing program (Image J). The resulting Red-Green-Blue (RGB) color intensity is directly proportional to the concentration of cholinesterase enzyme. Ellman's method was used to test the accuracy of the analytical method. Then, field testing was conducted among 40 farmers from Na Mai subdistrict, Lat Lum Kaeo District, Pathum Thani Province. The colorimetric pad provided a linear dynamic range between 0.01-2.0 µg/ml with a correlation coefficient (R2) of 0.9832. The results of Ellman's method analysis revealed an R2 value equal to 0.9892. The screening results with a paper-based device found that 95 percent of farmers had the cholinesterase enzyme in their blood between 0.39 to 1.97 µg/ml. The development of this device could serve as a tool for screening the health risks of farmers who work with pesticides.
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