The Level of Memory B Cells (CD27+ CD19+) in Patient with Neuromyelitis Optica Spectrum Disorder with Positive AQP4-IgG Antibody

Abstract

Neuromyelitis Optica Spectrum Disorder (NMOSD) is a central nervous system inflammatory disorder. One part of pathogenesis of disease is related to abnormal B cells function and cytokine expression especially B cells sub population called memory B cells (CD27 + CD19 +). The objective of this study is to compare the percentage of memory B cells between patients diagnoses with NMOSD and healthy subject control. The secondary objective is to compare percentage of memory B cells between the private laboratory and the laboratory of Institute of Pathology, Department of Medical Services. The blood from ten patients with NMOSD and equal number of healthy subject control were analyzed as percentage of memory B cell (CD27+ CD19+) by using flow cytometry. The percentage of memory cells were compared between patients with NMOSD and healthy subject control. Moreover the result of memory B cell from commercial private laboratory was compared with laboratory form Institute of Pathology, Department of Medical Services. The result shown percentage of memory B cells (CD27+ CD19+) in patients with NMOSD was significance higher than healthy subject control. The percentage difference of memory cell was 2.54% (p=0.028). In patients with recent relapse, the percentage of memory B cells was significance higher than non-relapse groups which 5.71% higher (p=0.003). There was no significance difference percentage of memory cells across private laboratory and laboratory of Institute of pathology, Department of medical service (p=0.064).