Modified TB Fast Amp to Finding Negative Acid Fast Stain in Tuberculosis’ Patient during 2013-2017: Reports of Pathological Laboratory of Regional Medical Science Center 10th Ubon Ratchathani
Keywords:Tuberculosis, Negative acid fast stain, TB FastAmp
Tuberculosis is an infectious disease that continues to be a public health problem in many countries around the world. In 2015, it is the second leading cause of death in the world. 1.8 million people died from the disease and 10.4 million have been infected. The improvement in patient monitoring and patient screening, as well as patients with smear-negative tuberculosis is important. By 2014, the World Health Organization reports that only 63 per cent (6 million) of the 9.6 million people were detected. It means that 37 per cent of all tuberculosis patients are unlikely to be detectable. The aim of this study was to identify smear-negative tuberculosis patients with using TB Fast Amp. 385 patients were reported from Public Health Laboratory of Medical Science Center 10 Ubon Ratchathani during 2013 to 2017. The positive results were 59 cases, 15.32%. Negative results were 326 cases, 84.68% 5-years old minimum, 91-years old maximum, 204 males, 52.99%, 181 females, 47.01%. There were 25 home-related patients, 17.24% and 120 no home-sickness, 82.76%. 35.8 cases had previous tuberculosis treatment, 24.82%. 106 cases had no previous tuberculosis treatment, 75.18%. The results from the TB FastAmp study could help to find out negative-smear tuberculosis patients 15.32%. Then this molecular technique should be used for the detection of tuberculosis in clinical trials, especially in countries with limited resources.Keyword: Tuberculosis, Negative acid fast stain, TB FastAmp
MedThai. Tuberculosis; Symptoms, causes, treatment. [Cited 2017 Oct 19]; Available from: https://medthai.com/ Tuberculosis /
James McIntosh. Tuberculosis: Causes, Symptoms, andTreatments. [Cited 2017 Oct 19]; Available from http:// www.medicalnewstoday.com/articles/8856.php/Accessed July 20, 2017.
World Health Organization. Tuberculosis. [Cited 2017 Oct 19]; Available from: http://www.who.int/mediacentre/ factsheets/fs104/en/Accessed July 20, 2017.
Bureau of Policy and Strategy Ministry of Public Health. Border health strategy 2017-2023. [Cited 2017 Oct 13]; Available from: https//: bps.moph.go.th/new_bps/sites/ default/ files/1.%20 border_health_strategy.pdf
Labtestonline. AFB-Culture. [Cited 2017 Oct 13]; Available from: https://labtestsonline.org/understanding/analytes/ afb-culture/tab/test. Accessed July 20, 2017.
Silairat S. New technique for Tuberculosis diagnosis. [Cited 2017 Oct 14]; Available from: http://www.wongkarnpat. com/viewpat.php?id=1789#.WeRnmI-0PDc
Stop-TB Partnership. The global plan to stop TB 2011-2015: transforming the fight towards elimination of tuberculosis. Geneva: World Health Organization; 2010.
CDC. Update Guidelines for the use of nucleic acid amplification test in diagnosis of Tuberculosis. [Cited 2017 Oct 13]; Available from: www.cdc.gov/mmwr/preview/ mmwrhtml/mm5801a3.htm. Accessed August 20, 2017.
Rudeeaneksin J, BoonChu S, Tunchau H, Sawanpanyalert P, Kamolwat A . Fast tuberculosis examination using LAMP test, direct sputum examination and identification of the infection Growing up. Journal of Public Health, Vol. 19, No. 2 (March-April 2010), pages 300 - 310.
Kaewpinit T, Santiwattanakul S, Chansiri K. Loop-mediated Isothermal Amplification (LAMP) technique for Tuberculosis diagnosis. Journal of Medicine and Health Sciences (Vol. 20 No.1 April 2013) p. 13-20.
Khamsaen J, Panumaswiwat S, Chaipanna B, Ittitummaboon P, Kaewsri W, Jearakul W, et al. The result of using LAMP test helps detect TB patients in the negative smear group in Rasrisalai hospital, Sri Sa Ket and Det Udom Crown Prince Hospital, Ubon Ratchathani. The 35th Medical Technology Conference. April 26-29, 2011 at Sunee Hotel and Convention Center Ubon Ratchathani. (Poster presentation)
Khamsaen J, Teerawattanasuk N, Petsuksiri B, Rudeeaneksin J, Aramruang P, Jearakul W, et al. Use of LAMP test for diagnosis of ex-pulmonary tuberculosis In Sunpasitthiprasong Hospital Ubon Ratchathani. The 19th Congress of Medical Sciences, 17-19 August 2011, at the Miracle Grand Hotel, Nonthaburi Province. (Poster presentation)
Klayut W, Srisangngam S, Rudeeaneksin J, BoonChu S, Noppornphan K. Comparison of non-LAMP and LAMP test. Isothermal amplification, A new method for tuberculosis testing. [Cited 2017 Aug 25]; Available from: https://nih. dmsc.moph.go.th/KM/59/research2559/poster/wipat.pdf
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