Cyclooxygenase-2- inhibiting activity of polyphenols in extracts of tea from various geographic locations in Thailand


  • Kansiri Topothai, D.D.S. Institute of Dentistry, Department of Medical Services
  • Nyampak Tayana, M.Sc Drug Discovery and Development Center, Office of Advance Science and Technology, Thammasat University
  • Saisuree Prateeptongkum, Dr. rer. Nat. Department of Chemistry, Faculty of Science and Technology, Thammasat University
  • Nongnaphat Duangdee, Dr.rer.nat Drug Discovery and Development Center, Office of Advance Science and Technology, Thammasat University
  • Esthera Prateeptongkum, D.D.S., Dip,, Institute of Dentistry, Department of Medical Services


Green tea, Camellia sinensis, inflammation, polyphenols, cyclooxygenase-2 (COX-2)


Background:Polyphenols are essential compounds for anti-inflammation commonly found in green tea (Camellia sinensis). They influence the inflammatory process by controlling and inhibiting pro-inflammatory cytokines such as cyclooxygenase-2 (COX-2) which is a prominent substance in dental diseases namely periodontal inflammation and oral cancer. However, their quantitative levels of polyphenols depend on geographical locations.Objectives: The aim of this research is to determine the polyphenol levels, important compounds for Cyclooxygenase-2 (COX-2) inhibition, of green tea extracted from various geographical locations in Thailand.Method: 70% Ethanol extraction samplings of green tea products were done in groups representing each province in Thailand. The chemotype levels of polyphenols was determined by total polyphenols (Folin-Ciocalteu method) and total flavonoid. Catechin-specific epigallocatechin gallate (EGCG) level was then measured by high-performance liquid chromatography (HPLC). Result: Total polyphenols, total flavonoids, and EGCG content in green tea from each province in Thailand are significantly different (p<0.05).  The highest amount of polyphenols and flavonoids is Chiang Rai tea. The highest amount of EGCG is Chiang Mai tea. Anti-inflammatory test results resembled as IC50 are ranked in descending order from Chiang Rai, Assam, Narathiwat and Chiang Mai green tea which corresponding to the amount of important substances in green tea, but there is no statistically significant difference (p< 0.05).Conclusion: Chemotype levels of polyphenols in green tea vary in different cultivation areas ranked in descending order from Chiang Rai province (Assum tea), Narathiwat province and Chiang Mai Province. The chemotype levels of polyphenols    exhibiting preliminary anti-inflammatory properties correlate directly to the concentration of tea. Accordingly, it is shown that green tea grown in Thailand statistically significantly exhibits anti-inflammatory properties.


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How to Cite

Topothai K, Tayana N, Prateeptongkum S, Duangdee N, Prateeptongkum E. Cyclooxygenase-2- inhibiting activity of polyphenols in extracts of tea from various geographic locations in Thailand. j dept med ser [Internet]. 2022 Mar. 31 [cited 2022 Oct. 1];47(1):119-28. Available from:



Original Article