Comparison of the Glass Pipette and RAMA SP Pipette for Sperm Preparation

Main Article Content

Anna Wongkularb
Sarin Ammarit
Wichan Choktanasiri

Abstract

Objective: To evaluate the DNA fragmentation on human sperm assessed by Ramathibodi’s SP pipette.


Methods: In this experimental study, thirty samples of freshly ejaculated human semen having a sperm density 20 million per mL or more and a percentage of motile sperm 50 percent or more were used in this study. The percentage of sperm motility was evaluated within 30 minutes after ejaculation. After semen analysis, the semen was divided into two parts, one part was kept as a control (assessed by glass pipette) and the other used for the experiment (assessed by Ramathibodi’s SP pipette).


Results: After sperm preparation, the DNA fragmentation with Ramathibodi’s SP pipette was observed to decrease from 21.1 to 9.8 while that of the control (assesses by glass pipette) decreased to 10.2.


Conclusions: Our study indicates that DNA fragmentation of human sperm by using Ramathibodi’s SP pipette is superior to that of the glass pipette.

Article Details

How to Cite
Wongkularb, A., Ammarit, S., & Choktanasiri, W. (2013). Comparison of the Glass Pipette and RAMA SP Pipette for Sperm Preparation. Ramathibodi Medical Journal, 36(4), 264–268. Retrieved from https://he02.tci-thaijo.org/index.php/ramajournal/article/view/117602
Section
Original Articles

References

Meseguer M, Santiso R, Garrido N, García-Herrero S, Remohí J, Fernandez JL. Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. Fertil Steril. 2011;95(1):124-128. doi:10.1016/j.fertnstert.2010.05.055.

Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod. 2010;25(7):1594-1608. doi:10.1093/humrep/deq103.

World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Sperm Cervical Mucus Interaction, 4th. Cambridge University Press, Cambridge, UK. 1999.

Domínguez-Fandos D, Camejo MI, Ballescà JL, Oliva R. Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy. Cytometry. 2007;71(12):1011-1018.

Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21(1):33-44.

Benchaib M, Braun V, Lornage J, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod. 2003;18(5):1023-1028.

Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A. Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J Androl. 2006;8(1):11-29.