The Study of the Yield of DNA Extracted From Hairs of Postmortem Cases

Main Article Content

Kanchana Sujirachato
Suranan Tirawatnapong
Nurhasnee Mimae
Sirirat Inon
Adisuk Kaewdouengdee
Wisarn Worasuwannarak

Abstract

Background: Problems frequently occur from hair extraction are low concentration and impurity of DNA.


Objective: To assess the Phenol/Chloroform/Isoamyl alcohol method for extracting DNA from hair roots.


Methods: Hairs from 30 postmortem cases were collected. In each case, 5 and 10 hair roots were extracted DNA by Phenol/Chloroform/Isoamyl alcohol. The amount and purity of DNA (A260/280) were detected by NanoDrop spectrophotometer. The quality of DNA was examined using polymerase chain reaction (PCR). Amount of DNA was analyzed compared with gender, age, and manner of death.


Results: The amount and purity of DNA extracted from 10 hair roots were slightly better than 5 hair roots (P > .05). The mean (range) of DNA concentration was 200.4 (35.2 - 799.6) ng/µL from 10 hair roots vs 148.2 (21.7 - 571.5) ng/µL from 5 hair roots in 30 µL volume whereas the mean (range) of A260/280 was 1.61 (1.31 - 1.99) in 10 hair roots vs 1.54 (1.32 - 1.90) in 5 hair roots. The results from NanoDrop showed no difference among various gender, age, and manners of death.


Conclusions: DNA can be extracted from hair by Phenol/Chloroform/Isoamyl alcohol. The quantity and quality of DNA were good enough for personal identification by PCR. DNA extracted from 10 hair roots is better than 5 hair roots.


 

Article Details

How to Cite
Sujirachato, K., Tirawatnapong, S., Mimae, N., Inon, S., Kaewdouengdee, A., & Worasuwannarak, W. (2020). The Study of the Yield of DNA Extracted From Hairs of Postmortem Cases. Ramathibodi Medical Journal, 43(2), 9 - 18. https://doi.org/10.33165/rmj.2020.43.2.224635
Section
Original Articles

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