Evaluation of 3 Common Methods for Effective Housefly Allergen Extraction

Main Article Content

Chonvara Chalermrujinanant
Panwadee Pluangnooch
Kitipong Soontrapa

Abstract

Background: Allergen extracts have been applied to treat allergic diseases. Accordingly, a housefly (Musca domestica) extract is commonly used to treat patients severely allergic to housefly.


Objective: To evaluate 3 common methods, including grinding, sonication, and homogenization, for effective preparation of housefly allergen extracts.


Methods: Housefly allergens were extracted from Musca domestica using 3 different methods, including grinding, sonication, and homogenization. Protein concentrations and profiles in the extracts were determined by Bradford assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively.


Results: The protein concentrations of the extracts prepared by grinding (mean [SD], 911.3 [159.7] µg/µL) and sonication (mean [SD], 905.7 [188.6] µg/µL) as measured by Bradford assay were significantly higher than those prepared by homogenization (mean [SD], 674.5 [60.0] µg/µL). Moreover, SDS-PAGE showed more protein bands in the extracts prepared using grinding and sonication compared to those prepared using homogenization.


Conclusions: In comparison to homogenization, both grinding and sonication methods are superior ways to prepare housefly allergen extracts as evidenced by the higher quantities and composition of proteins.


 

Article Details

How to Cite
1.
Chalermrujinanant C, Pluangnooch P, Soontrapa K. Evaluation of 3 Common Methods for Effective Housefly Allergen Extraction. Rama Med J [Internet]. 2021 Mar. 26 [cited 2024 Mar. 29];44(1):40-5. Available from: https://he02.tci-thaijo.org/index.php/ramajournal/article/view/245935
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Original Articles

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