MicroRNA Expression of Primary and Metastatic Colorectal and Breast Carcinoma

Thaniya Sricharunrat; Artit Jinawath; Pattana Sornmayura; Sansanee Wongwaisayawan; Budsaba Rerkamnuaychoke

doi:10.33165/rmj.2022.45.3.257343

Authors

Thaniya Sricharunrat Pathology and Forensic Science Department, Chulabhorn Hospital, Chulabhorn Royal Academy, Bangkok, Thailand
Artit Jinawath Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Pattana Sornmayura Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Sansanee Wongwaisayawan Department of Medical Science, Faculty of Science, Rangsit University, Pathum Thani, Thailand
Budsaba Rerkamnuaychoke Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

DOI:

https://doi.org/10.33165/rmj.2022.45.3.257343

Keywords:

MicroRNA, Breast cancer, Colorectal carcinoma, Metastasis

Abstract

Background: Colorectal and breast carcinoma are frequently diagnosed cancers. At advanced stages, cancers metastasize to certain organs resulting in loss of function of these organs, and eventually death. Therefore, there is a specific need for the prognosis of these cancers. Currently, microRNAs (miRNAs), have emerged as a new target of cancer-specific biomarker.

Objective: To examine the expression of miRNA in primary and metastatic breast and colorectal cancers.

Methods: This study investigated the expression of 6 miRNAs (miR-10b, miR-21, miR-145, miR-155, miR-200c, and miR-373) in formalin fixed paraffin embed tissues from pairs of normal tissues with primary and metastatic tumor samples of breast and colorectal carcinoma cases in Ramathibodi Hospital, Thailand by real-time RT-PCR.

Results: Among 6 miRNAs, miR-145 decreased significantly in all samples of primary and metastatic colorectal and breast carcinoma. There was significantly decreased expression of miR-145 in metastatic colorectal carcinoma compared to their primary colorectal carcinoma (P < .05). Whereas miR-10b, miR-155, and miR-200c showed a decreased expression; miR-21, and miR-373 showed an increased expression in the majority of cases. Unlike miR-145, other miRNAs showed no significant difference of expression (P > .05).

Conclusions: This finding indicates that miR-145 may be the potential metastatic biomarker. Decrease of miR-145 could be applied to the prognosis and target for therapy of breast and colorectal carcinoma.

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