Correlations Among Hyaluronan Binding Assay, Annexin-V/Propidium Iodide Staining and Acrosome Reaction for Sperm Quality Assessment

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Surachai Dejarkom
Suwanthana Yamthanod
Warinda Phoonthaweerat
Kwansuda Supalap
Patcharada Amatyakul


Objective: To evaluate correlation among Hyaluronan Binding Assay (HBA), Annexin-V binding and Acrosome Reaction (AR) with semen analysis by World Health Organization (WHO) criteria.
Material & Methods: Twenty-seven infertile men underwent semen analysis at the infertility center, Naresuan university hospital using basic quantitative method by WHO criteria and qualitative methods by HBA, Annexin-V binding and AR. The results were calculated for correlations among these methods.
Results: The mean age of the study group was 34.2 ± 5.4 years old. The semen analysis showed normal volume, sperm concentration, progressive motility, total motility and morphology. The result of HBA was 87.5%. According to the annexin-V binding assay, most of sperm (80.47%) was viable without apoptosis. The results of Sperm Spontaneous Acrosome Reaction Rate (SARR) and acrosome reaction after ionophore challenge were within normal ranges. There was no correlation between HBA and sperm parameters evaluated by WHO criteria. However, HBA bound significantly correlated with viable sperm and HBA bound negatively correlated with late apoptotic sperm (rs 0.466, p = 0.014 and -0.563, p = 0.002, respectively).
Conclusion: Hyaluronic acid binding assay was correlated with viable sperm and low late apoptosis, but not correlated with sperm acrosome reaction.


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Dejarkom S, Yamthanod S, Phoonthaweerat W, Supalap K, Amatyakul P. Correlations Among Hyaluronan Binding Assay, Annexin-V/Propidium Iodide Staining and Acrosome Reaction for Sperm Quality Assessment. Siriraj Med Bull [Internet]. 2022 Apr. 1 [cited 2023 Dec. 6];15(2):70-7. Available from:
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