Laboratory Tests for the Diagnosis of Onychomycosis

Main Article Content

Chatisa Panyawong
Charussri Leeyaphan
Sumanas Bunyaratavej
Lalita Matthapan
Waranyoo Prasong
Wittawat Turakit
Punyawee Ongsri

Abstract

Onychomycosis is a fungal infection of the nail plate or nail apparatus. Onychomycosis is commonly found in populations in tropical countries and in the elderly. Onychomycosis cause unusual nail appearance and discoloration. Most people with onychomycosis commonly had several predisposing factors such as frequent hand washing, contacting to soil environment, poor hygienic nail or foot care. The most common causative organisms of onychomycosis in Thailand are dermatophytes, followed by non-dermatophytes and yeast. Normally, fungi are invisible to the naked eye but it can be detected by mycological laboratory, for example, KOH examination, fungal culture, and histopathological examinations. Laboratory examinations are very important in diagnosing fungal nail infection and making differentiate diagnosis from other nail diseases such as traumatic onychodystrophy, psoriatic nails. Nowadays, prevalence of onychomycosis is increasing, so correct diagnosis should be emphasized to lead to the effective and safety treatment. After recovering from onychomycosis, some patients may have a chance of recurrent disease. Patients should be aware of hand-foot health care by keeping their hands and feet clean and dry. Finding shoes with appropriate arch support will increase walking comfort. Socks should be washed after each wear and stored in a clean, dry place, which can help avoid foot infections or fungus growth.

Article Details

How to Cite
1.
Panyawong C, Leeyaphan C, Bunyaratavej S, Matthapan L, Prasong W, Turakit W, Ongsri P. Laboratory Tests for the Diagnosis of Onychomycosis. Siriraj Med Bull [Internet]. 2023 Jul. 1 [cited 2024 Dec. 4];16(3):256-2. Available from: https://he02.tci-thaijo.org/index.php/simedbull/article/view/260857
Section
Review Article

References

Gupta AK, Jain HC, Lynde CW, Macdonald P, Cooper EA, Summerbell RC. Prevalence and epidemiology of onychomycosis in patients visiting physicians’ offices: a multicenter Canadian survey of 15,000 patientsexternal icon. J Am Acad Dermatol. 2000;43:244-8.

Bunyaratavej S, Pattanaprichakul P, Leeyaphan C, Chayangsu O, Bunyaratavej S, Kulthanan K. Onychomycosis: A study of self-recognition by patients and quality of life. Indian J Dermatol Venereol Leprol. 2015;81:270-4.

Repetto EC, Giacomazzi CG, Castelli F. Hospital-related outbreaks due to rare fungal pathogens: a review of the literature from 1990 to June 2011. Eur J Clin Microbiol Infect Dis. 2012;31:2897-904.

Dyanne P, Michael J. Onychomycosis: Current trends in diagnosis and treatment. Am Fam Physician. 2013;88(11):762-70.

Bunyaratavej S, Srinonprasert V, Kiratiwongwan R, Wongdama S, Leeyaphan C. Onychomycosis in older : The age and associated factors affecting the complete cure rate. Australas J Dermatol. 2022;63(1):74-80.

Thomas J, Jacobson GA, Narkowicz CK, Peterson GM, Burnet H, Sharpe C. Toenail onychomycosis: an important global disease burden. J Clin Pharm Ther. 2010;35(5):497-519.

Assadamongkol R, Lertwattanarak R, Wannachalee T, Bunyaratavej S, Leeyaphan C, Matthapan L. Prevalence, Risk Factors, and Type of Organism in Fungal Foot Infection and Toenail Onychomycosis in Thai Diabetic Patients. J Med Assoc Thai. 2016;99:659-64.

Surjushe A, Kamath R, Oberai C, et al. A clinical and mycological study of onychomycosis in HIV infection. Indian J Dermatol Venereol Leprol. 2007;73(6):397-401

Gupta AK, Stec N, Summerbell RC, Shear NH, Piguet V, Tosti A, Piraccini BM. Onychomycosis: a review. J Eur Acad Dermatol Venereol. 2020;34:1972-90.

Wolff K, Goldsmith L, Katz S, Gilchrest B, Paller AS, Leffell D. Fitzpatrick's Dermatology in general medicine 7th ed. MeGraw-Hill. 2008:1806-10.

Wolff K, Goldsmith L, Katz S, Gilchrest B, Paller AS, Leffell D. Fitzpatrick's Dermatology in general medicine 8th ed. MeGraw-Hill. 2012:1013-14.

Salakshna N, Bunyaratavej S, Matthapan L, Lertrujiwanit K, Leeyaphan C. A cohort study of risk factors, clinical presentations and outcomes for dermatophyte, non-dermatophyte and mixed toenail infections. J Am Acad Dermatol. 2018;79:1145-6.

Bunyaratavej S, Limphoka P, Kiratiwongwan R, Leeyaphan C. Survey of skin and nail fungal infections by subject age among Thai adults and the etiological organisms. Southeast Asian J Trop Med Public Health. 2019;50:1118-30.

Lipner SR, Scher RK. Onychomycosis: Clinical overview and diagnosis. J Am Acad Dermatol. 2019;80:835-51.

Lipner SR, Scher RK. Onychomycosis: Treatment and prevention of recurrence. J Am Acad Dermatol. 2019;80:853-67.

Elewski B, Charif M. Prevalence of onychomycosis in patients attending a dermatology clinic in Northeastern Ohio for other conditions. Arch Dermatology. 1997;133:1172-3.

S, Bunyaratavej S, Leeyaphan C, Rujitharanawong C, Surawan TM, Muanprasat C, Atthapan L. Efficacy of 5% amorolfine nail lacquer in Neoscytalidium dimidiatum onychomycosis. J Dermatolog Treat. 2016;27:359-63.

Leeyaphan C, Bunyaratavej S, Prasertworanun N, Muanprasart C, Matthapan L, Rujitharanawong C. Dermatophytoma: An under-recognized condition. Indian J Dermatol Venereol Leprol. 2016;82:188-9.

Singal A, Khanna D. Onychomycosis: Diagnosis and management. Indian J Dermatol Venereol Leprol. 2011;77(6):659-72.

Finch J, Arenas R, Baran R. Fungal melanonychia. J Am Acad Dermatol. 2012;66(5):830-41.

Luplertlop N, Suwanmanee S. Dermatophytosis: from bench to bedside. J Trop Med Parasitol. 2013;36:75-87

Pumirat P, Tunyong W, Luplertlop N. Medical Mycology. J Med Health SCI. 2013;20(2):31-44.

Kondori N, Tehrani PA, Strömbeck L,Faergemann J. Comparison of Dermatophyte PCR Kit with Conventional Methods for Detection of Dermatophytes in Skin Specimens. Mycopathologia. 2013;176(3-4):237-41.

Luplertlop N, Suwanmanee S. Dermatophytosis: from bench to bedside. J Trop Med Parasitol. 2013;36:75-87.

Matthapan L, Leeyaphan C, Limphoka P, Lertrujiwanit K, Prasong W, Bunyaratavej S. J Med Asso Thai. 2021;104(3):383-87.

Odds FC. Sabouraud(‘s) agar. J Med Vet Mycol. 1991;29(6):355-9.

Li XF, Shen YN, Chen W, Chen H, Lv GX,Liu WD. A new medium for diagnosis of dermatophyte infection. Eur J Dermatol. 2009;19(1):34-7.

Ruan E, Johan K, Carolina H. Candida albicans or Candida dubliniensis?. Mycoses. 2011;54(1):1-16.

Shemer A, Davidovici B, Grunwald H, Trau H, Amichai B. New criteria for the laboratory diagnosis of nondermatophyte moulds in onychomycosis. Br J Dermatol. 2009;160(1):37-9.