Antimicrobial Susceptibility of Fosfomycin Polymethylmethacrylate Beads after sterilization
Keywords:
Fosfomycin, polymethylmethacrylate beads, ethylene oxide, sterilization, Methicillin-sensitive Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA), E. coli, Enterococcus sp, Pseudomonas aeruginosaAbstract
Objective: Fosfomycin Polymethylmethacrylate beads (FPMMA) can be used to treat bone infection. After each use, there was some FPMMA waste. If it can be kept in sterile condition for later use. It will safe time and cost. The idea of the study is to compare the antimicrobial susceptibility to Methicillin-sensitive Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA), E. coli, Enterococcus sp. and Pseudomonas aeruginosa of FPMMA before and after sterilization with ethylene oxide gas. The study design is experimental study. (laboratory research)
Methods: Mixed fosfomycin with PMMA and moulded into beads. All FPMMA 20 beads were equally divided into two groups: control and experimental groups. The experimental group was sterilized again with ethylene oxide gas before testing. The antimicrobial susceptibility was identifed by density meter in Mcfarland unit for both groups. The result was recorded and compared.
Results: Mean turbidity of control group in broth with MSSA, MRSA, E. coli, Enterococcussp. and Pseudomonas aeruginosa were 0.21+0.02, 0.58+0.04, 1.14+0.10, 0.32+0.03 and 0.36+0.02respectively in experimental group MSSA, MRSA, E. coli, Enterococcus sp. and Pseudomonasaeruginosa were 0.02+0.01, 0.18+0.02, 1.20+0.12, 2.6+0.25 and 0.22+0.02 respectively. Statisticalanalysis demonstrated signifcant difference between two groups.(p-value< 0.05)
Conclusion: The antimicrobial susceptibility to MSSA, MRSA, and Pseudomonas aeruginosa were not less than the new FPMMA beads but E. coli and Enterococcus sp. were less than the new FPMMA beads after sterilization with ethylene oxide gas.
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