Physical Properties and Anti-Bacterial Activity of Essential Oils Extracted from Kaffir Lime, Lemongrass, and Lime

Main Article Content

Firdao Hengta
Umniyah Kuna
Yamon Pitakpawasutth
Sukanjana Kamlungmak

Abstract

Plants produce essential oils naturally in different parts. Essential oil from herb plants are used as massage oil, medicine, skin care products, Inhalers, and food ingredients. The objectives of this research were 1) to study the physical characteristics, 2) to assess the amount of distilled essential oil (% yield), 3) to evaluate the distillation cost of essential oil, and 4) to study the antibacterial activity. The results of the study found that the physical characteristics of essential oils from the kaffir lime leaf were yellow gold and had a pungent smell, lemongrass was light yellow and had a particular scent, and lime peel was yellow gold and had a particular scent and pH values were 3.74, 3.74, and 3.94, respectively. The essential oil yields were 0.37%, 0.23%, and 0.32%, respectively. The cost of essential oils per 1 mL was 11.59, 11.13, and 14.02 baht, respectively. Kaffir lime leaf essential oil at concentrations of 0.10-0.18% v/v, lemongrass essential oil at 0.10% v/v, and lime peel essential oil at concentrations ranging from 0.18% to 1.56% v/v demonstrated antibacterial characteristics and were effective to inhibit Staphylococcus aureus, Streptococcus pyrogens, and Streptococcus pneumonia. Hence, it is suitable to use the potential of these three essential oils to use in formulations and commercial products for future research.

Article Details

How to Cite
1.
Hengta F, Kuna U, Pitakpawasutth Y, Kamlungmak S. Physical Properties and Anti-Bacterial Activity of Essential Oils Extracted from Kaffir Lime, Lemongrass, and Lime. Health Sci J Thai [Internet]. 2024 Oct. 25 [cited 2024 Nov. 21];6(4):57-64. Available from: https://he02.tci-thaijo.org/index.php/HSJT/article/view/267600
Section
Original articles

References

Chaichana C, Wongkalasin S, Manotuang D. The analysis of suitable area in thai herb planting for support of Thai herbal medicine at luang pu faep supinyo hospital: a case study of Sakon nakhon province. Humsu 2021; 40(4): 32-46. (In Thai)

Luang-In V, Aromatherapy from essential oils. BUSCIJ 2018; 23(1): 61-78. (In Thai)

Pipatpaiboon N, Mayod T, Nuphan P, Huchaiyaphum N, Meesang M, Srichamnan S. Comparison volume of essential oils of fresh and dried kaffir lime leaves from the distillation of essential oils with a 30 liter distiller. J. Agric. Sci. Technol. 2022; 3(1): 12-22. (In Thai)

Jirukakul P. Research and development on components natural oil extractor with supercritical carbon dioxide technique (sc-co2) and combination mixer magnetic. [Internet]. 2023 [Cited in 9 October, 2023]. Available from:https://www.doa.go.th/research/attachment.php?aid=2584

Maymam K, Poungtat S, Sakulsaowapakkul N, Maymam K, Nateesinsub K, Bunroek P. The comparison of inhibiting efficiency of Staphylococcus aureus by three types of essential oils: lemongrass, kaffir lime leaves, and Centella asiatica (Linn.) Urban. Suan Sunandha Meeting 9th National Academic Conference on Upgrading Research to Innovatio 2021; 1(1): 43-52. (In Thai)

Ekpenyong CE, Akpan E, Nyoh A. Ethno-pharmacology, phytochemistry, and biologi-cal activities of Cymbopogon citratus (DC.) Stapf extracts. Chin J Nat Med 2015; 13(5): 321–37.

Monatrakulsreepian P. Antibacterial Activities of essential oils from the plants Citrus spp. against methicillin-resistant Staphylococcus aureus. Rangsit University Research 2021. (In Thai)

Announcement regarding minimum continuous matters (No.10) Gazette. Announcement and general edition 2019. Volume 136, Special Section 316. [Internet]. 2023 [Cited in 9 October, 2023]. Available From: https://shorturl.asia/Qx3LO

Jittabut P, Tooklang P, Liawklang S, Bamrungna D. The system extracting essential oil from herbal plants by using rice husk as fuel. STBRU 2020; 4(2): 65-79. (In Thai)

Botanicessence. Pure essential oils. [Internet]. 2024 [Cited in 24 June, 2024]. Available from: https://botanicessence.com/essential-oil/home/products.jsp?range=eo&screen=1&sort=rank&sys=2023-01-01

Strategy and Information Group, Phatthalung provincial agricultural extension office. Basic agricultural information on plants, economic insects, groups, centers, and farmer institutes. Phatthalung Province. [Internet]. 2023 [Cited in 9 October, 2023]. Available from: https://www.opsmoac.go.th/phatthalung-dwl-files-451191791792?fbclid=IwAR2Zi_eUELXhXPQ1K7r_wDv5KZNPkDAEXSUPT5O39bCaoE5j32D8Kcz0Kq8

Department of Thai Traditional and Alternative Medicine. [Internet]. 2024 [Cited in 24 June, 2024]. Available from: https://abdul.dtam.moph.go.th/thaiherbs/herb_pdf/0167.pdf?fbclid=IwZXh0bgNhZW0CMTAAAR2oaKKuu_XKuCj8Dc5WROBYNwz6jnm3kF9czvclI0nQGN-gJy_fxvMnRIw_aem_djcNE6lpJbLARn45vJNiPA

Perry N, Perry E. Aromatherapy in the management of psychiatric disorders clinical and neuropharmacological perspectives. CNS Drugs 2006; 20(4): 257-280.

Jimbo D, Kimura Y, Taniguchi M, Inoue M, Urakami K. Effect of aromatherapy on patients with Alzheimer's disease. Psychogeriatrics 2009; 9(4): 173-179.

Smith CA, Collins CT, Crowther CA. Aromatherapy for pain management in labor. Cochrane Database Syst. Rev. 2011; 6(7): CD009215.

Lai TK, Cheung MC, Lo CK, Ng KL, Fung YH, Tong M, Yau CC. Effectiveness of aroma massage on advanced cancer patients with constipation: a pilot study. Complement. Ther. Clin. Pract. 2011; 17(1): 37-43.

Celeiro M, Guerra E, Lamas JP, Lores M, Garcia-Jares C, Llompart M. Development of a multianalyte method based on micro-matrix-solid-phase dispersion for the analysis of fragrance allergens and preservatives in personal care products. J. Chromatogr. A. 1344 (2014): 1-14.