Antioxidant and antibacterial activities of Butea monosperma (Lam.) Taub. flower extracts
Article Sidebar
Main Article Content
Abstract
Butea monosperma (Lam.) (commonly known as the flame of the forest or Palash) is a plant that has been used in traditional medicine for a long time. It has been reported to contain various bioactive compounds such as flavonoids, phenolics, and tannins, which possess antioxidant properties. These compounds help neutralize free radicals and reduce oxidative stress, making the plant a subject of interest for its potential health benefits and therapeutic applications. In the present study, total phenolic compounds, antioxidant activities, and antibacterial activities of the hydroethanolic extract from the flower of B. monosperma were evaluated. The total phenolic compound was quantified based on the Folin–Ciocalteu method, and the antioxidant activities of the extracts were assessed through the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The broth dilution method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were used to evaluate the antibacterial activity of the extracts against Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis and, Escherichia coli. The results revealed that the total phenolic content of the extracts was 65±0.014 mg of gallic acid equivalent/g of extract. Additionally, the antioxidant activities in terms of IC50 value of the extracts were 0.44±1.181 mg/ml and 16.054±0.188 mg/ml, according to the DPPH and the FRAP assay. Crude extracts of B. monosperma flower also demonstrated antibacterial activity against selected bacterial strains, with MIC and MBC values ranging from 1.56 to 200 mg/mL. In summary, the results suggest that flower extracts of B. monosperma may serve as valuable sources of bioactive compounds with antioxidant and antibacterial properties. However, further research involving the isolation and purification of these bioactive compounds is necessary to elucidate their mechanism of action.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Zhang Y-J, Gan R-Y, Li S, Zhou Y, Li A-N, Xu D-P, et al. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules. 2015;20(12):21138–56.
Nowak D, Gośliński M, Kłębukowska L. Antioxidant and antimicrobial properties of selected fruit juices. Plant Foods Hum Nutr. 2022;77(3):427–35.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007;70(3):461–77.
Abbasi S, Gharaghani S, Benvidi A, Latif A. Identifying the novel natural antioxidants by coupling different feature selection methods with nonlinear regressions and gas chromatography-mass spectroscopy. Microchem J. 2018;139:372–9.
Goswami N, Chatterjee S. Assessment of free radical scavenging potential and oxidative DNA damage preventive activity of Trachyspermum ammi L. (carom) and Foeniculum vulgare Mill. (fennel) seed extracts. Biomed Res Int. 2014;2014:582767.
Bardaweel SK, Tawaha KA, Hudaib MM. Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil. BMC Complement Altern Med. 2014;14(1):297.
Islam MZ, Hossain MT, Hossen F, Mukharjee SK, Sultana N, Paul SC. Evaluation of antioxidant and antibacterial activities of Crotalaria pallida stem extract. Clin Phytoscience. 2018;4(1). doi.org/10.1186/s40816-018-0066-y
Zhang Z, Gao L, Cheng Y, Jiang J, Chen Y, Jiang H, et al. Resveratrol, a natural antioxidant, has a protective effect on liver injury induced by inorganic arsenic exposure. Biomed Res Int. 2014;2014:617202.
Devi WR, Singh SB, Singh CB. Antioxidant and anti-dermatophytic properties leaf and stem bark of Xylosma longifolium clos. BMC Complement Altern Med. 2013;13(1):155.
Afsar T, Khan MR, Razak S, Ullah S, Mirza B. Antipyretic, anti-inflammatory and analgesic activity of Acacia hydaspica R. Parker and its phytochemical analysis. BMC Complement Altern Med. 2015;15(1):136.
Ninkaew S, Chantaranothai P. The Genus Butea Roxb. Ex Willd. (Leguminosae-Papilionoideae) in Thailand. Chiang Mai J. Sci. 2015; 42(2) : 367-375.
Somani R, Kasture S, Singhai AK. Antidiabetic potential of Butea monosperma in rats. Fitoterapia. 2006;77(2):86–90.
Choedon T, Shukla SK, Kumar V. Chemopreventive and anti-cancer properties of the aqueous extract of flowers of Butea monosperma. J Ethnopharmacol. 2010;129(2):208–13.
Rana F, Avijit M. REVIEW ON BUTEA MONOSPERMA. Ijrpc.com. [cited 2024 May 7]. Available from: https://www.ijrpc.com/files/19-2180.pdf
Moonpa R, Suntornptthue A, Visutthi M. Ethanolic Extract of Piper betle L. Leaves for Controlling of Bovine Mastitis. Science and Technology Research Journal Nakhon Ratchasima Rajabhat University 2020; 5(2): 22-29. (in Thai)
Buachoon N, Kookid S. Total phenolic and antioxidant activity of mango cv. nam dokmaiin sa kaeo province. VRU Research and Development Journal Science and Technology 2019; 14: 121-132. (in Thai)
Kaisoon O, Siriamornpun S, Weerapreeyakul N, Meeso N. Phenolic compounds and antioxidant activities of edible flowers from Thailand. J Funct Foods. 2011;3(2):88–99.
Phaiboon N, Pulbutr P, Sungthong B, Rattanakiat S. Effects of the ethanolic extracts of guava leaves, licorice roots and cloves on the cariogenic properties of streptococcus mutans. Pharmacogn J. 2019;11(5):1029–36.
Torrungruang K. Antibacterial activity of mangosteen pericarp extract against cariogenic Streptococcus mutans. Chulalongkorn University Dental Journal. 2007 [cited 2024 May 7];30(1):1–10. Available from: https://digital.car.chula.ac.th/cudj/vol30/iss1/1/
Farahmandfar R, Tirgarian B, Dehghan B, Nemati A. Comparison of different drying methods on bitter orange (Citrus aurantium L.) peel waste: changes in physical (density and color) and essential oil (yield, composition, antioxidant and antibacterial) properties of powders. J Food Meas Charact. 2020;14(2):862–75.
Pintathong P, Chomnunti P, Sangthong S, Jirarat A, Chaiwut P. The feasibility of utilizing cultured Cordyceps militaris residues in cosmetics: Biological activity assessment of their crude extracts. J Fungi (Basel). 2021;7(11):973.
Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006;99(1):191–203.
Baessa M, Rodrigues MJ, Pereira C, Santos T, da Rosa Neng N, Nogueira JMF, et al. A comparative study of the in vitro enzyme inhibitory and antioxidant activities of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret from Pakistan: New sources of natural products for public health problems. S Afr J Bot. 2019;120:146–56.
Tawaha K, Alali F, Gharaibeh M, Mohammad M, Elelimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem. 2007;104(4):1372–8.
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, et al. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules. 2022;27(4):1326.
Sungthong B, Phadungkit M. Anti-tyrosinase and DPPH radical scavenging activities of selected Thai herbal extracts traditionally used as skin toner. Pharmacogn J. 2015;7(2):97–101.
Chokchaisiri R, Suaisom C, Sriphota S, Chindaduang A, Chuprajob T, Suksamrarn A. Bioactive flavonoids of the flowers of Butea monosperma. Chem Pharm Bull (Tokyo). 2009 [cited 2024 May 8];57(4):428–32.
Liu J, Li X, Cai R, Ren Z, Zhang A, Deng F, et al. Simultaneous study of anti-ferroptosis and antioxidant mechanisms of butein and (S)-butin. Molecules. 2020;25(3):674.
Boulekbache-Makhlouf L, Slimani S, Madani K. Total phenolic content, antioxidant and antibacterial activities of fruits of Eucalyptus globulus cultivated in Algeria. Ind Crops Prod. 2013;41:85–9.
Karnwal A. In vitro antibacterial activity of Hibiscus rosa sinensis, Chrysanthemum indicum, and Calendula officinalis flower extracts against Gram negative and Gram positive food poisoning bacteria. Advances in Traditional Medicine. 2022;22(3):607–19.
Dave KM, Darji PP, Gandhi FR. Antimicrobial activity and phytochemical study of plant parts of Butea monosperma. J Drug Deliv Ther. 2019;9(4-A):344–8.
