Pigmented Rice Extracts Inhibit Calcium Oxalate Crystal Formation and Adhesion to MDCK Renal Tubular Cell Line
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Abstract
Kidney stone is a worldwide chronic disease with a recurrence rate ranging from 21–25%. Natural antioxidant-rich substances, particularly plant extracts such as lemon, green tea, and okra, have been explored for their potential in preventing kidney stone formation. This study aimed to investigate the effects of Black glutinous rice extract (BR) and Sung-Yod glutinous rice extract (SY) on calcium oxalate crystal nucleation, aggregation in vitro, and crystal adhesion to MDCK renal tubular epithelial cells. The statistical analyses were performed using a one-way analysis of variance with a significance level of P < 0.05. The results demonstrated that BR and SY extracts significantly inhibited calcium oxalate crystal formation and aggregation in a dose-dependent manner. Furthermore, treatment with BR and SY extracts reduced the number of crystal-MDCK cell adhesions. It can be concluded that rice extracts, particularly those from black glutinous rice, inhibit calcium oxalate crystal formation and adhesion in MDCK renal tubular cells. It contributes to the development of potential preventive agents against kidney stones. Further studies are warranted to elucidate the underlying mechanisms by which these rice extracts exert their anti-lithiatic effects.
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