Elevated Urinary 8-hydroxy Deoxyguanine Levels as a Marker of Oxidative DNA Damage in Type 2 Diabetes Mellitus Patients

บทคัดย่อ

Objective: To investigate the elevation of urinary 8-Hydroxydeoxygaunosisne (8-OHdG), as a biomarker of oxidative DNA damage in Type 2 diabetes patients(T2D) and the association with the conventional cardiovascular risk factors.

Methods: We examine the level of urinary 8-OHdG and the other blood biochemical markers in both 51 T2D patients [58.0 (53.0-65.0) yrs] and 61 healthy controls [63.0 (54.0-68.0) yrs]. Urinary 8-OHdG was determined by a competitive ELISA method. Serum malondialdehyde (MDA) was determined as thiobarbituric acid reactive substance (TBARS) as a marker of lipid peroxidation.

Results: Both serum levels of MDA and urinary 8-OHdG was significantly higher in T2D patients than healthy controls (P<0.001). Glucose level was significantly correlated with TC, TG levels and inverse correlated with HDL-C levels (p<0.05). Urinary 8-OHdG was significantly correlated with MDA, TC and LDL-C (p<0.05).

Conclusion: Our results showed that oxidative DNA damage and oxidative stress were occurred in T2D patients. These may be the good biomarker for oxidative stress event that may implicate in various pathological complication conditions in T2D patients.