Study of Antioxidant Activity and Alpha-glucosidase Inhibitory Activity of Herbal Extracts of Thai Traditional Medicine Formulas of Mor Chit Bunluang Wang Wiset District, Trang Province
Keywords:
Medicine Formulas, Antioxidant Activity, Alpha-Glucosidase InhibitoryAbstract
Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Symptoms often include polydipsia, polyphagia, weight loss, and glycosuria. This study investigated the antioxidant activity and α-glucosidase inhibitory effects of a traditional Thai herbal formula used by a local herbalist to treat diabetes for over 20 years. The formula consists of seven herbs: 1 .Piper sarmentosum Roxb. 2. Lagerstroemia speciosa Pers. 3. Pluchea indica (L.) Less. 4. Zea mays (L). 5. Caryota mitis Lour. 6. Scoparia dulcis (L.) and 7. Drynaria quercifolia (L.) J.Sm. Both aqueous and ethanolic extracts of the formula were evaluated for their antioxidant activity using the DPPH assay and α-glucosidase inhibitory activity. The results showed that both extracts exhibited significant antioxidant activity, with the aqueous extract demonstrating a slightly higher IC50 value, 10.31 ± 0.01 μg/ml, compared to the ethanolic extract, 16.71 ± 0.01 μg/ml. Similarly, both extracts significantly inhibited α-glucosidase activity, with the aqueous extract showing an IC50 value of 79.17 ± 0.01 μg/ml and the ethanolic extract showing an IC50 value of 70.32 ± 0.94 μg/ml. These findings suggest that the traditional Thai herbal formula possesses potent antioxidant and α-glucosidase inhibitory properties, supporting its potential as a functional food or nutraceutical for diabetes management. Further studies are warranted to elucidate the underlying mechanisms and to evaluate the formula's efficacy and safety in clinical settings.
References
Amria, M. M., Nermin, M. E., Abeer, H. A., Nayera, A., Wagdy, K. B., Mahmoud, N., Amani, N. H., Elsayed, A. E., & Sherien, K. H. (2019). Lagerstroemia Speciosa (L.) Pers Leaf Extract Attenuates Lung Tumorigenesis via Alleviating Oxidative Stress, Inflammation and Apoptosis. Biomolecules, 9(12), 871. http://doi.org/10.3390/biom9120871
Arfan, A., Alif, K., On-Anong, S., Monthon, L., & Gornganok, P. (2022). Antioxidant and Antimicrobial Activities of Leaves Crude Extracts of Lagerstroemia speciosa (L.) Pers. Princess of Naradhiwas University Journal, 14(2), 165 - 176. https://li01.tci-thaijo.org/ index.php/pnujr/article/view/253110
Campos, A.M., Sotomayor, C.P., Pino, E., & Lissi, E. (2004). A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols. A Comparison with closely related Methodologies. Biological Research 37(2), 287 - 92 http://doi.org/ 10.4067/s0716-97602004000200015
Choudhury, B. (2022). Effect of Lagerstroemia Speciosa (L.) Pers. (Jarula) Leaves Powder as a Hypoglycemic Agent in the Management of Type 2 Diabetes Mellitus: An Interventional Study. Ayuhom 8(2), 64 - 67. http://doi.org/10.4103/AYUHOM.AYUHOM_4_22
Department of Disease Contro, Ministry of Public Health. (2021, March 1). Incidence and prevalence ates of hypertension, diabetes, coronary artery disease, cerebrovascular disease, and COPD patients from 2020 to 2022. http://www/thaincd.com/2016/mission/documents-detail.php?id=13684&tid=32&gid=1-020.
Gerich, J.E., Meyer, C., Woerle, H.J. & Stumvoll, M. (2001). Renal gluconeogenesis: its importance in human glucose homeostasis. Diabetes Care. 24(2), 382-391.
Joanna, J., Orejola, J.V., Toralba, E.A.C., & Ladignon. (2015). Alpha-glucosidase Inhibitory Effect of Crude Extracts from Selected Philippine Medicinal Plants. Orejola. 19(4), 1-12. https://pjhrd.upm.edu.ph/index.php/main/article/view/49
Pareek, A., Suthar, M., Garvendra, S., Rathore, Bansal, V., & Kumawat, T. (2010). In Vitro Antioxidant Studies of Lagerstroemia speciosa Leaves. Pharmacognosy Journal, 2(10), 357-360. http://doi.org/10.1016/S0975-3575(10)80109-9
Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 1-9. http://doi.org/10.1016/j.ijbiomac.2023.123470
Rossella, C., Vincenzo, G., & Aldo, E.C. (2023). Anti-Dyslipidemic and Anti-Diabetic Properties of Corosolic Acid: A Narrative Review. Endocrines. 4, 616 - 629. http://doi.org/10.3390/ endocrines4030044
Suttithumsatid, W., Shah, M.A., Bibi, S., & Panichayupakaranant, P. (2022). α-Glucosidase inhibitory activity of cannabidiol, tetrahydrocannabinol and standardized cannabinoid extracts from Cannabis sativa. Current Research in Food Science, 5, 1091-1097. http://doi.org /10.1016/j.crfs.2022.07.002
Saad Alkahtani, Md Saquib Hasnain, Hamzah Algamdy, Nada H. Aljarba, Abdullah AlKahtane. (2022). Acute and sub-acute oral toxicity Lagerstroemia speciosa in Sprague-Dawley rats. Saudi Journal of Biological Sciences 29 (2022) 1585–1591.
The International Diabetes Federation. (2023). IDF Diabetes Atlas, 10th Edition 2021. https://diabe tesatlas.org
Tran, V.C., To, D. C., Phan, T.Q., Thanh, Q. B., Le, V.T., Nguyen, V. H., Nguyen, T.T., Duc, V. H., Nguyen, C.B., Nguyen, T.A.N. (2022). Antioxidant activity and α‐glucosidase inhibitability of Distichochlamys citrea M.F. Newman rhizome fractionated extracts: in vitro and in silico screenings. Chemical Papers, 76, 5655 - 5675. http://doi.org/10.1007/s11696-022-02273-2
Wanwisa, K., Passakorn, B., Ronnarit, K., Achida, J., Benjamart, C., Sakulrat, R., & Pawitra, P. (2018). Inhibitory Actions of Lagerstroemia speciosa (L.) Pers. Aqueous and Ethanolic Leaf Extracts against Carbohydrate-digesting Enzymes. Pharmacognosy Journal, 10(6), 113-118. http://doi.org/10.5530/pj.2018.6s.22
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