Study molecular sequence typing of Neisseria gonorrhoeae in Bangrak STIs Center with Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) method
DOI:
https://doi.org/10.14456/taj.2021.5Keywords:
Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST), Sequence type (ST)Abstract
Gonorrhea has been caused from “Neisseria gonorrhoeae” which is one of five common sexually
transmitted diseases and a major issue in many countries around the world. According to the report of WHO 2018, there are new patients in United State of America 87,000 cases per year which had increased more than 5.70% since 2015. Similarly, in Thailand, the trend of gonorrhea was increased during the same
period. In 1935, Antibiotic have been used for gonorrhea treatment. Nowadays third-generation of Cephalosporin has used in patient but there were a decrease of susceptibility in 3rd Cephalosporin. This study was supported bacteria by Division of AIDS and STD those provide samples which stored during October to November 2016 to use in the experiment. A total samples of 98 gonococcal isolates were analyzed for antibiotic susceptibility test and examine the genetic (Sequence type: ST) for Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST). As the result, the experiments showed that Ciprofloxacin, Tetracycline and Penicillin were decreasing susceptibility until resistance, while Azithromycin, Cefixime and Ceftriaxone were not resistance but there found 1 sample present a decrease in susceptible trend. The result of NG-MAST was 28 different result, representing 46.00%. By NG-MAST method, STs relation was not found which reported drug resistance in any countries. This study of ST could be benefit for sequence typing for the trend of multidrug resistance. Especially, the risk groups or tourist should be monitored for the spread drug resistance control.
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