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The aim of this research was to study the effect of the development of nursing practice guideline for the prevention of pressure injury of the high-risk patients at Ranong Hospital, Ranong province. The subjects, who were 112 professional nurses and high-risk patients of pressure injury group aged more than 60 years old who have not had no pressure injury, were recruited. The criteria of assessment were the patients who have Barden Scale £18 points. Based on the Barden Scale criteria, 697 patients were admitted in 10 wards during September 2021-February 2022. Data were analyzed by using descriptive and comparative statistics. After applied the developed practice guideline in professional nurses, the results showed that they have significantly higher knowledge (p=0.001) for the preventing pressure injury of high- risk patients. When compared with the target average score of the preventing pressure injury of high-risk patients that was fixed at 75%, the results showed that the average of their attitude was significantly higher than the target average score (p=0.001). The rate of pressure injury in high-risk patients was decreased from 5.22 to 2.80 per 1000 days (length of stay in high-risk patients) when compared to that of before the development of nursing practice guideline. The results indicated that the success of the development of nursing practice guideline for the prevention of pressure injury of high-risk patients of pressure injury at Ranong Hospital, Ranong province
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