ระบาดวิทยาของซีโรทัยป์เชื้อไวรัสเดงกี่ ในเขตภาคกลางตอนล่าง

บทคัดย่อ

          Dengue hemorrhagic fever is one of most important infectious diseases in every part of Thailand. Dengue virus occurs as four distinct serotypes, each of which causes epidemics throughout the tropical and subtropical regions. Many studies have examined co-circulation of multiple dengue virus serotypes in population over time and their capacity to produce severe dengue diseases. In this paper report the relation of dengue serotypes and clinical diagnosis, population characteristic and severe dengue diseases. Dengue diagnosis is usually made by serology such as enzyme linked immunosorbent assay (ELISA), but serological techniques do not identify the infecting strain. The reverse transcription polymerase chain reaction (RT-PCR) can detect and identify all four serotypes of dengue viruses simultaneously in nested procedure. We used IgM & IgG capture ELISA to detect dengue virus by using the plasma of 656 patients with clinical diagnosis of dengue in Lower central part of Thailand in April 2002 to March 2003, and used RT-PCR method to isolated dengue virus. A total of 100 dengue virus isolates were obtained from acute dengue virus infection ; 8.0% were DEN-1, 49.0% were DEN-2, 6.0% were DEN-3, 23.0% were DEN-4 and 14.0% could not identified. In conclusion of this study, dengue virus can infect in higher age population and can find all dengue serotypes in lower Central part of Thailand, although DEN-2 produced more severe symptoms than the other dengue serotypes.