The Monitoring on Potentially Pre – cancerous Changes Prevalence in Positive HPV E6/E7 mRNA Subjects in Chiang Mai Province Using Pap Smear Method

Abstract

Cervical cancer is a significant public health problem among married women which requires a sensitive screening method. Human papilloma viruses E6/E7 mRNA (HPV E6//E7 mRNA) is proposed as a potential marker to indicate potentially pre-cancerous changes before pathological alteration. In 2012, we compare HPV E6//E7 mRNA prevalence in 100 sex workers to conventional pap smear positive prevalence. We found 5 subjects with positive pap smear and 33 subjects with positive HPV E6/E7 mRNA. We did not found any statistic relation between these methods. However, HPV E6/E7 mRNA possibly an early detection of pre-cancerous changes before it is detected by using pap smear method. Objective: We monitored the prevalence of pre-cancerous changes using pap smear among 33 subjects who showed HPV E6/E7 mRNA positive in 2012 survey compared to those in 33 subjects with negative HPV E6/E7 mRNA. Method: Liquid based cervical cytology specimens were collected at The Office of Diseases and Prevention Control 10 Chiang Mai. The pap smear was processed at Department of Pathology, Faculty of Medicine, Chiang Mai University at month 6 and month 12. Chi-square was use to compare the prevalence of pap smear positive between HPV E6/E7 mRNA positive and negative groups. Result and Conclusion: We did not find any pap smear positive subjects in both positive and negative HPV E6/E7 mRNA groups. The relation between HPV E6/E7 result and pap smear result could not observed in this study. The follow up period in this study to observe changing of the tissue among HPV E6/E7 mRNA subjects take 1 year but the changing of the tissue from normal to be cancer tissue take the different time during 2 to 10 years. Therefore, we suggest to extend the follow up period for determination of a cervical cancer prevalence among HPV E6/E7 mRNA positive subjects.