Phytochemical screening and antibacterial activity of Combretum quadrangulare kurz. extracts

Authors

  • jarinya khoontawad คณะทรัพยากรธรรมชาติ มหาวิทยาลัยเทคโนโลยีราชมงคลอีสาน วิทยาเขตสกลนคร
  • Chatlada Hongwilai
  • Sirintip Promsensa
  • Naruwat Pakdee
  • Panicha Pongnaratorn
  • Pongsathorn Tongkasee
  • Chatchanok Nukulkit
  • Thanthika Kaewsoongnern
  • Pensiri Chan-ae

Keywords:

Combretum quadrangulare Kurz, Phytochemical, Antibacterial activity, Staphylococcus aureus

Abstract

Objective : To study phytochemical screening and antibacterial activity on Staphylococcus aureus from fresh and dry parts of the branches, barks, fruits and leaves of Combretum quadrangulare Kurz.

Method : Analytic study by experimental research from fresh and dried parts from branches, barks, fruits and leaves extracted with 95% ethanol for phytochemical screening and antibacterial activity against Staphylococcus aureus by Disc diffusion method and  Broth dilution method. The statistics were determined by One-Way ANOVA.

Results : The results of phytochemical screening of fresh and dried parts from branches, barks, fruits and leaves extracts showed different phytochemicals. However, flavonoids, saponins and tannins were found in all parts. The antibacterial activity on S. aureus were tested by using disk diffusion method.  The results showed that S. aureus were inhibited by the herbal extracts. The dried extracts had the better antibacterial performance than the fresh part. The highest antibacterial activity was found in dried part of branches extracts that have inhibition zone diameter of 20.00±0.50 mm, Minimal inhibitory concentration (MIC) of 1.95 mg/mL, Minimal bactericidal concentration (MBC) of 3.91 mg/mL while the highest antibacterial effect in fresh extracts are fruits that showed the inhibition zone diameter of 15.00±0.00 mm, MIC of 1.95 mg/mL, MBC of 3.91 mg/mL, respectively.

Conclusion : The results of this study show that C. quadrangulare Kurz. have the ability in antibacterial. This knowledge may be developed into herbal product in the future.

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Published

2022-08-29

How to Cite

khoontawad, jarinya, Hongwilai, C. ., Promsensa, S. ., Pakdee, N. ., Pongnaratorn, P. ., Tongkasee, P. ., Nukulkit, C. ., Kaewsoongnern, T. ., & Chan-ae, P. . (2022). Phytochemical screening and antibacterial activity of Combretum quadrangulare kurz. extracts. Mahasarakham Hospital Journal, 19(2), 14–23. retrieved from https://he02.tci-thaijo.org/index.php/MKHJ/article/view/254311

Issue

Vol. 19 No. 2 (2022): May-August

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Articles

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