Efficiency evaluation of an in-house real time PCR assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens at sexually transmitted infections clinic.
DOI:
https://doi.org/10.14456/taj.2021.2Keywords:
Chlamydia trachomatis, Neisseria gonorrhoeae, In-house real time PCR assayAbstract
Bangrak STIs Center provides routine service for C. trachomatis and N. gonorrhoeae detection by
using in-house real time PCR assay. There are various types of specimens, including urethral swab, cervical
swab, rectal swab and throat swab. Currently, a urine specimen is another specimen for testing because it’s easy to collect with self-collection process and it doesn’t make patients pain. The objective of this study was to evaluate the efficiency of in-house real time PCR assay in the detection of these two pathogens compared with APTIMA Combo 2 Assay for CT/NG from 100 urine samples. Together with the study to compare the efficiency of in-house real time PCR assay, APTIMA Combo 2 Assay for CT/NG and Gram stain method for N. gonorrhoeae detection. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy were analyzed. The comparative cost-effectiveness of the reagent and material used for C. trachomatis and N. gonorrhoeae detection by nucleic acid amplification testing was also assessed.
In-house real time PCR assay showed all 100% correlation agreement with APTIMA Combo 2 Assay for CT/NG. The result of N. gonorrhoeae comparison with culture method showed both in-house real
time PCR assay and APTIMA Combo 2 Assay for CT/NG also had all 100% correlation agreement. Meanwhile Gram stain showed sensitivity, specificity, positive predictive value, negative predictive value and accuracy 80%, 100%, 100%, 96% and 96.55%, respectively. The cost-effectiveness of in-house real time PCR for CT/NG was 252 bath/test while the commercial kit was 718 bath/test.
The in-house real time PCR assay showed acceptable performance characteristics in comparison with
the reference and the gold standard methods in all specimens tested with the cost-effectiveness more than 2.8 times. Therefore, our routine service for C. trachomatis and N. gonorrhoeae detection from urine specimen is effective and reliable. Help save budget on the purchase of analytical reagents and reduce the service cost result in more patient access in service unit. eading to better treatment and less transmission of pathogen to others.
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