The Detection of Seven Sexually Transmitted Infections in Women with Sexual Risk Behaviors Using the Multiplex Real-Time PCR Method
DOI:
https://doi.org/10.14456/taj.2023.3Keywords:
sexually transmitted infections (STIs), multiplex real-time polymerase chain reaction, female high-risk sexual behaviorAbstract
This cross-sectional study was conducted between January and June 2018, of which 100 symptomatic and asymptomatic participants were selected from the patients of sexual transmitted diseases clinic, Bangrak Medical Center, Division of AIDS and STIs. The participants were divided into two groups, the general women group (N=50) and the female sex worker group (N=50). An individual cervical swab was collected from each participant to test for seven sexually transmitted infections: Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Ureaplasma urealyticum (UU), Ureaplasma parvum (UP) and Trichomonas vaginalis (TV), using the multiplex real-time polymerase chain reaction method with AnyplexTM II STI-7 Detection. The results were analyzed using Seegene Viewer program. Descriptive statistics were applied to analyze the results. The findings revealed that Ureaplasma parvum had the highest rate of bacterial infection (48%), followed by Ureaplasma urealyticum (20%), Mycoplasma hominis (17%), Neisseria gonorrhoeae (11%), Chlamydia trachomatis (9%), Mycoplasma genitalium (6%). The rate of parasite infection was Trichomonas vaginalis (3%).There was no statistically significant difference between the two groups in terms of the seven sexual transmitted infections (p>0.05). However, the asymptomatic female sex worker group had a higher rate of these infections, indicating an increased risk of transmission. Therefore, screening tests for these seven sexually transmitted infections in female sex workers are needed for prevention and control of infection.
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