Quantitative Bactericidal Efficacy of Alcohol-Based Handrub in Thailand Using Modified EN 1276: 2009 Method
Keywords:
Alcohol-based handrub, hands disinfection, bactericidal efficacyAbstract
Alcohol-based handrub (ABHR) products are widely used to reduce hands contamination in Thailand due to their convenience and ease to use in areas where water is not available. In Thailand, ABHR bactericidal efficacy and its test method have not been established. In this study, European Standard EN 1276: 2009 was modified and validated to obtain suitable conditions. The modified method was validated at 34๐C with minimizing a test system volume. The bactericidal efficacy was determined at 1 minute contact time in the presence of Bovine Albumin Fraction V using 4 reference bacterial strains; Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, Enterococcus hirae ATCC 10541 and Pseudomonas aeruginosa ATCC 15442. The ABHR product was considered as bactericidal effective when log reduction (lg R) of the reference bacterial amount after one minute contact time was ≥ 5. Furthermore, twenty-nine ABHR products marketed in Bangkok Metropolitan Region were evaluated using the proposed method. It was found that 6 ABHR products (20.7%) failed the bactericidal efficacy criterion. The finding emphasized a need for regulatory consideration of bactericidal efficacy of the ABHR product as a supportive scientific data. In addition, regular post-marketing surveillance of ABHR should be undertaken to ensure the bactericidal effectiveness of the products.
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