Simultaneously Quantitative of Phthalates in Health Care Products by UPLC/DAD

Abstract

       Phthalates are a class of industrial chemicals comprising about 30 commercial products which mostly used as plasticizer to enhance the flexible and soften of plastic. Presently, phthalates are used in variety of health care products both cosmetic and household products, thus they are widespread to human exposure. Toxicities of phthalates are concerned due to their mutagenicity in vitro and induce reproductive and developmental health effects in animals. Now, Thailand’s the Ministry of Public Health is banning five of phthalates i.e. DBP, DMEP, DEHP, PIPP and BBP. However, we still lack of the situation data of phthalates presented in health care products. This study is for the development of simultaneously determination of seven phthalates by UPLC/DAD technique and validation the method following EURACHEM Guideline. The study Kinetex ^®C-18 column (1.7 μm, 2.1 × 50 mm) was used. The separation was gradient programmed of the ratio of acetronitrile and deionized water with flow rate 0.45 ml/min, column temperature 35 ^oC, λmax = 225 nm and running time of 8 min. The validation results showed a good selectivity of seven phthalates with resolution > 2, %RSD (n =10) of within and between run were 0.01 – 0.12% and 0.29 – 1.71%. LOD and LOQ were in the range of 0.0002 – 0.007 %w/w and LOQ 0.001 – 0.022 %w/w. Linearity of the method was in the range of 0.05 – 30 μg/ml with correlation coefficient, r ≥ 0.995. Accuracy was presented as %recovery wihtin 90 – 108. The precision was presented as % RSD of peak area which was less than < 3% (n=10). The results showed that this method was valid appropriately for determination of seven phthalates in health care products subjecting for consumer protection.