A Study on the Quality and Quantity of DNA from Dried Blood Spots in Forensic Case Work
DNA from Dried Blood Spots in Forensic Case Work
Keywords:
Dried blood spot on filter paper, DNA degradation, Forensic medicineAbstract
The Division of Forensic Medicine at Thammasat University Hospital collected dried blood spots (DBS) on Whatman filter paper from deceased individuals who had died within 24 hours. To reduce sample storage space in collecting biological evidence, sample spoilage and biodegradation, which frequently occur, this study was undertaken aiming to explore the DNA content and quality of 49 DBS specimens that had been kept for 1–5 years. The results showed that DNA concentrations as measured by 260/280 nm absorbances were 6.10−200.60 ng/μL with DNA purity of 1.04−1.56. In addition, as analyzed with real-time PCR, the DNA concentrations were 0.24−66.32 ng/μL. Other DNA quality parameters or indices were found: Degradation index, 1.000−5.020; Inhibition index, 7.266−34.882; Mixture index, 3.613−6.200, and Male degradation index, 0.500−1.901. In conclusion, DNA degradation values increased more over time during the period of 1–5 years, but the DNA quantity was sufficient for forensic human identification. Further development efforts should be made to create a method for effective DNA extraction and inhibitive substance elimination; then the DNA samples extracted from DBS filter paper would be better used in forensic medicine.
References
Rahikainen AL, Palo JU, de Leeuw W, Budowle B, Sajantila A. DNA quality and quantity from up to 16 years old post-mortem blood stored on FTA cards. Forensic Sci Int 2016; 261: 148-53.
Chaisomchit S, Wichajarn R, Chowpreecha S, Chareonsiriwatana W. A simple method for extraction and purification of genomic DNA from dried blood spots on filter paper. Southeast Asian J Trop Med Public Health 2003; 34(3): 641-5.
Choi EH, Lee SK, Ihm C, Sohn YH. Rapid DNA extraction from dried blood spots on filter paper: potential applications in biobanking. Osong Public Health Res Perspect 2014; 5(6): 351-7.
Clinical and Laboratory Standards Institute (CLSI). Blood collection on filter paper for newborn screening program; approved standard-sixth edition. CLSI document NBS01-A6. Wayne, PA: Clinical and Laboratory Standards Institute; 2013.
Lin Z, Suzow JG, Fontaine JM, Naylor EW. A simple automated DNA extraction method for dried blood specimens collected on filter paper. JALA: J Assoc Lab Autom 2005; 10(5): 310-4.
Hue NT, Chan ND, Phong PT, Linh NT, Giang ND. Extraction of human genomic DNA from dried blood spots and hair roots. Int J Biosci Biochem Bioinforma 2012; 2(1): 21-6.
Fischer A, Lejczak C, Lambert C, Servais J, Makombe N, Rusine J, et al. Simple DNA extraction method for dried blood spots and comparison of two PCR assays for diagnosis of vertical human immunodeficiency virus type 1 transmission in Rwanda. J Clin Microbiol 2004; 42(1): 16-20.
Chathirat N. Predicting the UV spectrum of single stranded DNA. Appl Mech Mater 2015; 804: 163-6.
US 2014/0234834 A1. Method for quantifying human DNA. Patent Application Publication. [online]. 2014; [16 screens]. Available from: URL: https://patentimages.storage.googleapis.com/fe/3b/41/28b4f9b2bdcbe5/US20140234834A1.pdf.
Lee SB, McCord B, Buel E. Advances in forensic DNA quantification: a review. Electrophoresis 2014; 35(21-22): 3044-52.
Wilantho A, Mokmak W, Jenwitheesuk E. Genomics DNA extraction from clotted blood of cattle. KKU Vet J 2009; 19(1): 16-29.
St Julien KR, Jelliffe-Pawlowski LL, Shaw GM, Stevenson DK, O’Brodovich HM, Krasnow MA, Stanford BPD Study Group. High quality genome-wide genotyping from archived dried blood spots without DNA amplification. PLoS One 2013; 8(5): e64710. (7 pages).
Moretti TR, Moreno LI, Smerick JB, Pignone ML, Hizon R, Buckleton JS, et al. Population data on the expanded CODIS core STR loci for eleven populations of significance for forensic DNA analyses in the United States. Forensic Sci Int Genet 2016; 25: 175-81.
Kangsanan W, Somboonngern O, Pitileartpanya S, Pipatsatitpong D. Comparisons of DNA analysis using Qiagen® Investigator® 24plex QS kit and AmpFlSTR® Identifi ler® Plus PCR amplification kit. Science & Technology Asia 2020; 25(2): 75-85.
Nagata S. DNA degradation in development and programmed cell death. Annu Rev Immunol 2005; 23: 853-875.
Chadatong P, Lomthaisong K, Muisuk K. The effect of the sunlight on autosomal STR analysis of bloodstains on different types of clothes. In: The 19th National and international graduate research conference; 2018 March 9. Khon Kaen, Thailand: Khon Kaen University; 2018. p. 635-641.
Al-Kandari NM, Singh J, Sanger VC. Time-dependent effects of temperature and humidity on quantity of DNA in samples of human saliva, blood and semen in Kuwait. IJPSR 2016; 7(7): 2852-73.
Garvin AM, Holzinger R, Berner F, Krebs W, Hostettler B, Lardi E, et al. The forensiX evidence collection tube and its impact on DNA preservation and recovery. BioMed Res Int 2013; 2013: 105797. (7 pages).
Agetsuma-Yanagihara Y, Inoue E, and Agetsuma N. Effects of time and environmental conditions on the quality of DNA extracted from fecal samples for genotyping of wild deer in a warm temperate broad-leaved forest. Mamm Res 2017; 62: 201-7.
Wilfinger WW, Mackey K, Chomczynski P. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 1997; 22(3): 474-81.
Hares DR. Selection and implementation of expanded CODIS core loci in the United States. Forensic Sci Int Genet 2015; 17: 33-4.
Zahra A, Hussain B, Jamil A, Ahmed Z, Mahboob S. Forensic STR profiling based smart barcode, a highly efficient and cost effective human identifi cation system. Saudi J Biol Sci 2018; 25(8): 1720-3.
Fan GY, Wang DP, Song DL, Zheng XK, Zhu J, Long B. Developmental validation study of a 32-plex STR direct amplification system for forensic reference samples. Forensic Sci Int 2021; 327: 110977. (8 pages).
Phua CH, Kitpipit T, Pradutkanchana J, Duangsuwan P, Samai W, Thanakiatkrai P. Direct STR typing from human bones. Forensic Sci Int 2022; 330: 111099.
Xiao C, Yang X, Liu H, Liu C, Yu Z, Chen L, et al. Validation and forensic application of a new 19 X-STR loci multiplex system. Leg Med 2021; 53: 101957. (4 pages).
Emery MV, Bolhofner K, Winingear S, Oldt R, Montes M, Kanthaswamy S, et al. Reconstructing full and partial STR profiles from severely burned human remains using comparative ancient and forensic DNA extraction techniques. Forensic Sci Int Genet 2020; 46: 102272. (8 pages).
Hall CL, Kesharwani RK, Phillips NR, Planz JV, Sedlazeck FJ, Zascavage RR. Accurate profiling of forensic autosomal STRs using the Oxford Nanopore Technologies MinION device. Forensic Sci Int Genet 2022; 56: 102629. (10 pages).
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