Method Validation of Quercitrin Content in Houttuynia cordata Aqueous Extract Using Ultra High Performance Liquid Chromatography

Method Validation of Quercitrin in Houttuynia cordata Aqueous Extract Using UHPLC

Abstract

        Heartleaf (Houttuynia cordata Thunb.) is used for Thai traditional medicine and preparations registered by the Thai food and drug administration. It has been used for treating various symptoms such as fever, cough, and inflammation, and as diuretic. Additionally, it was reported as antiviral, antimicrobial, immune stimulant and anti-inflammatory. From Traditional Chinese Medicine, which heartleaf, as the main ingredient for allergic rhinitis during the COVID-19 pandemic, was reported. Phenolics were found as main compounds in this plant, of which quercitrin was reported as a major ingredient. Since the quality control of heartleaf aqueous extract has not been regulated, the objective of this study was to validate the method for analysis of the content of quercitrin in heartleaf aqueous extract Using Ultra High Performance Liquid Chromatography (UHPLC). The stationary phase was ZORBAX Extend-C18 Rapid Resolution HD 2.1x50 mm, 1.8 μm at a temperature of 30 ºC. The mobile phase was a mixture of 0.2 %v/v phosphoric acid in water and methanol as a gradient system. The flow rate was 0.4 mL/min. It was detected using photodiode array detector at 350 nm. The sample solution was prepared by refluxing with methanol for 30 minutes. The results showed that this method was specific. Linearity was between 0.04–0.24 mg/ml with r² = 0.99997. The accuracy (%recovery) was 99.76–103.16%. The HORRAT of repeatability and intermediate precision was less than 2. Limit of quantitation (LOQ) was 0.1 %w/w. The developed method was accurate and precise. Therefore, it can be used for the determination of quercitrin content as the quality control method of single heartleaf aqueous extracts which are commercially available.