Microbial Contamination of Injection After Opening in a Pediatric Ward

Abstract

          Pediatric doses of ampule-filled injections are generally less than adult doses. Divisions of such medicine for reuse purposes might cause microbial contamination leading to hazards to the sick children. The in situ use of these injections occurs in hospital wards despite the theroetical knowledge. These is no research article to reject this behavior, however. This study aims at investigating the effect of periodically opening of the injection ampules on the microbial contamination into the injection using nutrient broth at 37°C. The injections used are

aminophylline 250 mg. 10 c.c, sodium bicarbonate 3.75 gm. 50 c.c., terbutaline 0.5 mg. 1 c.c. and sterlie water for injection 10 c.c., After being cut-opened, micropore film is used to cover the openings of the ampules. Handling is done by aseptic techniques throughout the study. The injections are stored at 5°C and sampled every 6 hours for 24 hours for microbial count using UPS XXII method. The results, obtained from triplicate 3 samples each, show no bacterial growth in nutrient broth.