HBA as a Potential Alternative to the TUNEL Assay for Assessment of DNA Integrity in Asthenozoospermic Spermatozoa

Authors

  • Pitak Laokirkkiat Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Paweena Sarakarnkosol Taksin Hospital, Bangkok 10600
  • Isarin Thanaboonyawat Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Somsin Petyim Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Japarath Prechapanich Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Suphadtra Phornwilardsiri Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Noira Nigmatova Genome Astana, Astana
  • Roungsin Choavaratana Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Keywords:

Spermatozoa; asthenozoospermia; hyaluronan binding assay; TUNEL assay; infertility and DNA fragmentation

Abstract

Objective: This study was conducted to evaluate the appropriateness of the hyaluronan binding assay (HBA) for assessment of DNA integrity indirectly in asthenozoospermic spermatozoa, by studying its correlation with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay.
Methods: Thirty asthenozoospermic semen samples of infertile couples were recruited. Semen analysis was performed before being processed by HBA and the TUNEL assay. All the semen parameters were recorded and analyzed. 
Results:  The mean age of the patients was 38.1 ± 6.4 years. The average total and progressive motility was 33.5 ± 11.0% and 21.9 ± 6.6%, respectively. The mean TUNEL-positive and hyaluronan-bound sperm were 17.8 ± 6.4% and 57.2 ± 14.5%, respectively. Pearson correlation coefficient showed a high negative correlation between HBA and the TUNEL assay (r = -0.73, p < 0.001). The intraclass correlation coefficients revealed a high level of agreement between two observers who evaluated the TUNEL-positive and hyaluronan-bound sperm [0.81 (95% CI: 0.65–0.91) and 0.92 (95% CI: 0.84–0.96)], respectively.
Conclusion:  The study results showed a high negative correlation between HBA and the TUNEL assay. This suggests that HBA may be used as a viable intact DNA spermatozoa identification method, as a potential alternative to the TUNEL assay, in asthenozoospermia sperm. 

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Published

09-03-2018

How to Cite

Laokirkkiat, P., Sarakarnkosol, P., Thanaboonyawat, I., Petyim, S., Prechapanich, J., Phornwilardsiri, S., Nigmatova, N., & Choavaratana, R. (2018). HBA as a Potential Alternative to the TUNEL Assay for Assessment of DNA Integrity in Asthenozoospermic Spermatozoa. Siriraj Medical Journal, 70(1), 70–76. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/114910

Issue

Vol. 70 No. 1 (2018): Jan-Feb

Section

Original Article

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