Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital

Authors

  • Kanda Ekcharoenkul Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Popchai Ngamskulrungroj Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Keerati Joyjamras Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Amornrut Leelaporn Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Azian Harun Department of Medical Microbiology and Parasitology, School of Medical Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan
  • Pattarachai Kiratisin Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Abstract

Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology
laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method.
Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria.
Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica.
Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.

Downloads

Published

06-06-2018

How to Cite

Ekcharoenkul, K., Ngamskulrungroj, P., Joyjamras, K., Leelaporn, A., Harun, A., & Kiratisin, P. (2018). Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital. Siriraj Medical Journal, 70(2), 127–130. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490

Issue

Vol. 70 No. 2 (2018): March-April

Section

Original Article

License

Authors who publish with this journal agree to the following conditions:

Copyright Transfer

In submitting a manuscript, the authors acknowledge that the work will become the copyrighted property of Siriraj Medical Journal upon publication.

License

 Articles are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). This license allows for the sharing of the work for non-commercial purposes with proper attribution to the authors and the journal. However, it does not permit modifications or the creation of derivative works.

Sharing and Access

Authors are encouraged to share their article on their personal or institutional websites and through other non-commercial platforms. Doing so can increase readership and citations.