Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital

Article Sidebar

PDF
Published: Jun 6, 2018

Main Article Content

Kanda Ekcharoenkul
Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Popchai Ngamskulrungroj
Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Keerati Joyjamras
Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Amornrut Leelaporn
Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Azian Harun
Department of Medical Microbiology and Parasitology, School of Medical Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan
Pattarachai Kiratisin
Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Abstract

Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology
laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method.
Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria.
Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica.
Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.

Downloads

Download data is not yet available.

Article Details

How to Cite
Ekcharoenkul, K., Ngamskulrungroj, P., Joyjamras, K., Leelaporn, A., Harun, A., & Kiratisin, P. (2018). Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital. Siriraj Medical Journal, 70(2), 127–130. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490
Issue
Vol. 70 No. 2 (2018): March-April
Section
Original Article

All articles published in the Siriraj Medical Journal (SMJ) are protected by copyright. No material in this journal may be reproduced on any platform including electronic or in print or transmitted by any means, in whole or in part, without the prior written permission of the Editor of the SMJ. Written permission must also be obtained before any part of the SMJ is stored in any retrieval system of any nature.