Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital

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Kanda Ekcharoenkul
Popchai Ngamskulrungroj
Keerati Joyjamras
Amornrut Leelaporn
Azian Harun
Pattarachai Kiratisin

Abstract

Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology
laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method.
Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria.
Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica.
Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.

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How to Cite
Ekcharoenkul, K., Ngamskulrungroj, P., Joyjamras, K., Leelaporn, A., Harun, A., & Kiratisin, P. (2018). Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital. Siriraj Medical Journal, 70(2), 127–130. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490
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Original Article